| Texto completo | |
| Autor(es): |
Silva, J. C.
[1]
;
Ferreira-Strixino, J.
[1]
;
Fontana, L. C.
[1]
;
Paula, L. M.
[1]
;
Raniero, L.
[1]
;
Martin, A. A.
[1]
;
Canevari, R. A.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Vale Paraiba UNIVAP, IP&D, Lab Espectroscopia Vibrac Biomed, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Lasers in Medical Science; v. 29, n. 4, p. 1429-1436, JUL 2014. |
| Citações Web of Science: | 9 |
| Resumo | |
The aim of this study was to find the apoptosis molecular markers involved in the cell death that might be related to photodynamic therapy (PDT) mechanisms in breast cancer. The mammary tumors were induced in 25 Sprague-Dawley female rats by a single, oral gavage of 7,12-dimethylbenz(a)anthracene (DMBA; 70 mg/kg body weight). Animals were divided into four groups: G1 (normal, without DMBA), G2 (control, without PDT treatment), G3 (euthanized 48 h after PDT), and G4 (euthanized 24 h after PDT). For PDT experiments, the photosensitizer used was Photodithazine, and 100 J/cm of light at a fluence rate of 100 mW/cm was delivered to treat lesions. A sample of each animal was investigated by quantitative real-time PCR using Rat Apoptosis RT2 Profiler (TM) PCR Array platform. The results showed 20 genes with differential expression between PDT and control groups. A significant upregulation was observed for pro-apoptotic genes CASP4, CASP12, CIDEA, GADD45A, and FAS and downregulation of anti-apoptotic genes MAPK8IP1, TNFRSF11B, and NAIP2 in PDT-treated tumors. These results indicate that these genes are more directly involved in cell apoptosis induced by PDT. (AU) | |
| Processo FAPESP: | 10/00488-5 - Estudo dos mecanismos celulares envolvidos na terapia fotodinâmica |
| Beneficiário: | Juliana Ferreira Strixino |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/09523-0 - Análise da expressão dos genes relacionados a apoptose e a sua relação com a resistência celular após terapia fotodinâmica em carcinomas mamários |
| Beneficiário: | Jéssica Camila da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 09/18440-1 - Desenvolvimento de marcadores moleculares relacionados à resistência celular após terapia fotodinâmica em carcinomas mamários |
| Beneficiário: | Renata de Azevedo Canevari |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |