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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Apoptosis-associated genes related to photodynamic therapy in breast carcinomas

Full text
Author(s):
Silva, J. C. [1] ; Ferreira-Strixino, J. [1] ; Fontana, L. C. [1] ; Paula, L. M. [1] ; Raniero, L. [1] ; Martin, A. A. [1] ; Canevari, R. A. [1]
Total Authors: 7
Affiliation:
[1] Univ Vale Paraiba UNIVAP, IP&D, Lab Espectroscopia Vibrac Biomed, BR-12244000 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Lasers in Medical Science; v. 29, n. 4, p. 1429-1436, JUL 2014.
Web of Science Citations: 9
Abstract

The aim of this study was to find the apoptosis molecular markers involved in the cell death that might be related to photodynamic therapy (PDT) mechanisms in breast cancer. The mammary tumors were induced in 25 Sprague-Dawley female rats by a single, oral gavage of 7,12-dimethylbenz(a)anthracene (DMBA; 70 mg/kg body weight). Animals were divided into four groups: G1 (normal, without DMBA), G2 (control, without PDT treatment), G3 (euthanized 48 h after PDT), and G4 (euthanized 24 h after PDT). For PDT experiments, the photosensitizer used was Photodithazine, and 100 J/cm of light at a fluence rate of 100 mW/cm was delivered to treat lesions. A sample of each animal was investigated by quantitative real-time PCR using Rat Apoptosis RT2 Profiler (TM) PCR Array platform. The results showed 20 genes with differential expression between PDT and control groups. A significant upregulation was observed for pro-apoptotic genes CASP4, CASP12, CIDEA, GADD45A, and FAS and downregulation of anti-apoptotic genes MAPK8IP1, TNFRSF11B, and NAIP2 in PDT-treated tumors. These results indicate that these genes are more directly involved in cell apoptosis induced by PDT. (AU)

FAPESP's process: 10/00488-5 - Study of cellular mechanisms involved in photodynamic therapy
Grantee:Juliana Ferreira Strixino
Support Opportunities: Regular Research Grants
FAPESP's process: 11/09523-0 - Analysis of the expression of genes related to apoptosis and its relation to cellular resistance to photodynamic therapy after breast cancer
Grantee:Jéssica Camila da Silva
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 09/18440-1 - Development of molecular markers related to cellular resistance after photodynamic therapy in breast carcinomas
Grantee:Renata de Azevedo Canevari
Support Opportunities: Regular Research Grants