Role of hydrogen sulfide in the formalin-induced orofacial pain in rats

Autor(es)

Donatti, Alberto F. ^[1] ; Araujo, Rebeca M. ^[1] ; Soriano, Renato N. ^[1] ; Azevedo, Leopoldo U. ^[1] ; Leite-Panissi, Christie A. ^[1] ; Branco, Luiz G. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Morphol Physiol & Basic Pathol, Dent Sch Ribeirao Preto, BR-14040904 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: European Journal of Pharmacology; v. 738, p. 49-56, SEP 5 2014.
• Citações Web of Science: 13

Resumo

Hydrogen sulfide (H2S) is a gasotransmitter synthesized in peripheral tissues by the enzyme cystathionine gamma-lyase (CSE). This gas has been documented to be involved in a wide variety of processes including inflammation and nociception. The aim of the present study was to investigate the role of the peripheral H2S pathway in nociceptive response to the orolacial formalin experimental model of pain. Orofacial pain was induced by subcutaneous injection of formalin (1.5%, 50 mu l) into the upper lip of rats, and the time spent rubbing the face was measured at 3-min intervals for 45 min. Formalin induced a marked biphasic pain (first phase: 0-3 min; second phase: 15-33 min). Pretreatment with H2S donor (Na2S; 90 mu mol/kg), CSE inhibitor (propargylglycine; 26.5 and 88.4 mu mol/kg), or a preferential blacker of T-type Ca2+ channels (mibefradil; 028 and 2.81 mu mol/kg) attenuated the second phase of face rubbing when injected locally as well as systemically. Pretreatment with a selective blocker of K-ATP(+) channels (glybenclamide; 2.81 mu mol/kg) suppressed the Na2S-mediated attenuation of the formalin-induced pain second phase. Taken together these results suggest that endogenously produced H2S plays a pronociceptive role probably via T-type Ca2+ channels, whereas exogenous H2S exerts antinociceptive effects mediated by K-ATP(+) channels. (C) 2014 Elsevier B.V. All rights reserved, (AU)

• Processo FAPESP: 12/13888-7 - Avaliação do sulfeto de hidrogênio na nocicepção orofacial em ratos
• Beneficiário: Rebeca Miranda de Araújo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 12/01417-0 - Participação do sulfeto de hidrogênio (H2S) no bulbo ventrolateral rostral na modulação da resposta ventilatória à hipóxia
• Beneficiário: Alberto Ferreira Donatti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/12850-9 - Envolvimento do sulfeto de hidrogênio na hipotermia do choque endotoxêmico
• Beneficiário: Luiz Guilherme de Siqueira Branco
• Modalidade de apoio: Auxílio à Pesquisa - Regular