Mesenchymal Stem Cells Derived from Human Exfoliated Deciduous Teeth (SHEDs) Induce Immune Modulatory Profile in Monocyte-Derived Dendritic Cells

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Silva, Fernando de Sa ^{[1, 2, 3]} ; Ramos, Rodrigo Nalio ^[1] ; de Almeida, Danilo Candido ^[1] ; Bassi, Enio Jose ^[1] ; Gonzales, Roberto Pereira ^[1] ; Harumi Miyagi, Sueli Patricia ^[2] ; Maranduba, Claudineia Pereira ^[3] ; Brazil Esteves Sant'Anna, Osvaldo Augusto ^[3] ; Marques, Marcia Martins ^[4] ; Marzagao Barbuto, Jose Alexandre ^[1] ; Saraiva Camara, Niels Olsen ^[1] ; da Costa Maranduba, Carlos Magno ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Dent, Sao Paulo - Brazil

^[3] Univ Juiz Fora, Inst Biomed Sci, Juiz De Fora - Brazil

^[4] Butantan Inst, Immunochem Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 5 MAY 20 2014.
• Citações Web of Science: 5

Resumo

Background: Mesenchymal stem cells have prominent immune modulatory properties, which may have clinical applications; however their major source, bone marrow, is of limited availability. On the other hand, mesenchymal stem cells derived from human exfoliated deciduous teeth (SHEDs) are readily accessible, but their immune regulatory properties have not been completely investigated. This study was designed, therefore, to evaluate the SHEDs influence on DCs differentiation, maturation, ability to activate T cells and to expand CD4(+)Foxp3(+) T cells. Methodology/Principal Findings: The experiments were based in cellular co-culture during differentiation and maturation of monocyte derived-DCs (moDCs), with, or not, presence of SHEDs. After co-culture with SHEDs, (moDCs) presented lower expression of BDCA-1 and CD11c, in comparison to DC cultivated without SHEDs. CD40, CD80, CD83 and CD86 levels were also decreased in mature DCs (mDCs) after co-cultivation with SHEDs. To assess the ability of SHEDs-exposed moDCs to modulate T cell responses, the former were separated from SHEDs, and co-cultured with peripheral blood lymphocytes. After 5 days, the proliferation of CD4(+) and CD8(+) T cells was evaluated and found to be lower than that induced by moDCs cultivated without SHEDs. In addition, an increase in the proportion of CD4(+)Foxp3(+)IL-10(+) T cells was observed among cells stimulated by mature moDCs that were previously cultivated with SHEDs. Soluble factors released during co-cultures also showed a reduction in the pro-inflammatory cytokines (IL-2, TNF-alpha and IFN-gamma), and an increase in the anti-inflammatory molecule IL-10. Conclusion/Significance: This study shows that SHEDs induce an immune regulatory phenotype in moDCs cells, evidenced by changes in maturation and differentiation rates, inhibition of lymphocyte stimulation and ability to expand CD4(+)Foxp3(+) T cells. Further characterization and validation of this phenomenon could support the use of SHEDs, directly or indirectly for immune modulation in the clinical practice. (AU)

• Processo FAPESP: 10/09491-9 - Investigação da capacidade imuno-moduladora de células tronco imaturas de polpa dentária humana
• Beneficiário: Fernando de Sá Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado