Inhibitory effects of a cured antibacterial bonding system on viability and metabolic activity of oral bacteria

Autor(es)

Banzi, Efani C. F. ^[1] ; Costa, Ana R. ^[2] ; Puppin-Rontani, Regina M. ^[3] ; Babu, Jegdish ^[4] ; Garcia-Godoy, Franklin ^[5]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Pediat Dent Div, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dent Mat Div, BR-13414903 Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Piracicaba Dent Sch, Pediat Dent & Dent Mat Div, BR-13414903 Piracicaba, SP - Brazil

^[4] Univ Tennessee, Ctr Hlth Sci, Coll Dent, Dept Biosci Res, Memphis, TN 38163 - USA

^[5] Univ Tennessee, Ctr Hlth Sci, Coll Dent, Biosci Res Ctr, Dept Biosci Res, Memphis, TN 38163 - USA

• Tipo de documento: Artigo Científico
• Fonte: Dental Materials; v. 30, n. 9, p. E238-E244, SEP 2014.
• Citações Web of Science: 10

Resumo

Objective. To evaluate the antimicrobial efficacy of Clearfil SE Protect (CP) and Clearfil SE Bond (CB) after curing and rinsed against five individual oral microorganisms as well as a mixture of bacterial culture prepared from the selected test organisms. Methods. Bacterial suspensions were prepared from single species of Streptococcus mutans, Streptococcus sobrinus, Streptococcus gordonii, Actinomyces viscosus and Lactobacillus lactis, as well as mixed bacterial suspensions from these organisms. Dentin bonding system discs(6 mm x 2 mm) were prepared, cured, washed and placed on the bacterial suspension of single species or multispecies bacteria for 15, 30 and 60 min. MTT, Live/Dead bacterial viability (antibacterial effect), and XTT (metabolic activity) assays were used to test the two dentin system's antibacterial effect. All assays were done in triplicates and each experiment repeated at least three times. Data were submitted to ANOVA and Scheffe's f-test (5%). Results. Greater than 40% bacteria killing was seen within 15 min, and the killing progressed with increasing time of incubation with CP discs. However, a longer (60 min) period of incubation was required by CP to achieve similar antimicrobial effect against mixed bacterial suspension. CB had no significant effect on the viability or metabolic activity of the test microorganisms when compared to the control bacterial culture. CP was significantly effective in reducing the viability and metabolic activity of the test organisms. Significance. The results demonstrated the antimicrobial efficacy of CP both on single and multispecies bacterial culture. CP may be beneficial in reducing bacterial infections in cavity preparations in clinical dentistry. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/50414-8 - Uso de agentes antibacterianos na dentina desmineralizada e contaminada por S. mutans: análises biológica, morfológica e degradação da interface de união
• Beneficiário: Éfani Caroline de Freitas Banzi
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto