Comparative Analysis of the Spindle of Fresh In Vivo-Matured Human Oocytes Through Polarized Light and Confocal Microscopy: A Pilot Study

Autor(es)

Dib, Luciana Azor ^{[1, 2]} ; Da Broi, Michele Gomes ^{[1, 2]} ; Navarro, Paula Andrea ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Natl Inst Hormones & Womens Hlth, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Obstet & Gynecol, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTIVE SCIENCES; v. 21, n. 8, p. 984-992, AUG 2014.
• Citações Web of Science: 3

Resumo

Background: Considering the scarcity of literature data about the predictive capacity of polarization microscopy (PM) in identifying meiotic normality in human in vivo-matured oocytes, the main objective of the present study was to determine whether qualitative analysis of the spindle through PM can predict meiotic normality in fresh in vivo-matured human oocytes. Methods: Infertile patients undergoing controlled ovarian stimulation to intracytoplasmic sperm injection (ICSI) were selected. Fresh in vivo-matured oocytes were evaluated by PM and, immediately afterward, fixed for confocal microscopy (CM) analysis for evaluation of the spindle and chromosome distribution. We evaluated the percentage of oocytes with meiotic normality and abnormality determined by CM among oocytes with visible and nonvisible spindle and between oocytes with the spindle located at 0 degrees to 60 degrees angle to the first polar body (PB; normal position) and at 60 degrees to 90 degrees angle to the first PB (risky position) according to PM. Results: From 23 patients, 73 oocytes were analyzed. There were no significant differences in the percentage of oocytes with meiotic abnormalities among oocytes with visible and nonvisible spindles and among oocytes with the spindle in the normal and risky positions. Conclusions: Under the conditions tested, qualitative evaluation of the spindle through PM is not consistent with CM analysis and has limited predictive value of meiotic normality in fresh in vivo-matured human oocytes, which needs to be confirmed in larger studies. Our findings make questionable the usefulness of this methodology as a tool for noninvasive oocyte selection for ICSI. (AU)

• Processo FAPESP: 08/58197-6 - Balanço oxidante e antioxidante de mulheres inférteis com endometriose comparadas a controle (fatores masculino e tubário de infertilidade) submetidas a estimulação ovariana e influência nos resultados
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Regular