Via beta-adrenérgica e metabolismo energético de monócitos cd14+ de homens sedentarios ou treinados: impacto na polarização m1 e m2

• Processo: 24/20262-4
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de novembro de 2025
• Data de Término da vigência: 31 de outubro de 2028
• Área do conhecimento: Ciências da Saúde - Educação Física
• Pesquisador responsável: Fábio Santos de Lira
• Beneficiário: Fábio Santos de Lira
• Instituição Sede: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brasil
• Pesquisadores associados: Jonathan Peter Little ; José Cesar Rosa Neto ; Muhammad Hashim Islam ; Niels Olsen Saraiva Câmara
• Assunto(s): Exercício físico Imunometabolismo Metabolismo energético Obesidade
• Palavra(s)-Chave do Pesquisador: células imunológicas | exercício físico | Imunometabolismo | Metabolismo energético | miocinas | obesidade | Inflamação e metabolismo

Resumo

The association between sedentary behavior, physical inactivity and high caloric intake favors the accumulation of fat in the central region, increasing the risk of developing non-communicable chronic diseases. In addition, this condition affects the neuro-immune axis, impacting macrophage ¿-adrenergic signaling, and mitochondrial metabolism, favoring polarization toward a persistent inflammatory state. On the other hand, improvement in cardiorespiratory fitness (CRF) and reduction of body fat through physical training can promote positive modulations in the AR-¿2 pathway in the energy metabolism of macrophages, favoring polarization towards anti-inflammatory phenotypes. The aim of the present study is to explore the impact of the ¿2-adrenergic pathway on macrophage polarization in clinical and non-clinical conditions. The study will be divided into two stages: 1) individuals undergoing treatment with chronic ¿-blockers and their peers aged between 18 and 59 years will be recruited; 2) individuals aged between 18 and 40 years will be recruited and undergo central obesity assessments using ultrasound of the abdominal region, classified into LowVAT (¿3.1 cm) and HighVAT (¿5.1 cm), and an adjusting for cardiorespiratory fitness will be conducted. Blood samples will be collected to determine the metabolic and inflammatory profile, flow cytometry (CD14++CD16-, CD14++ CD16+ and CD14+ CD16++), LPS-stimulated whole blood culture (cytokine production). Furthermore, glucose uptake and energetic metabolism of CD14+ monocytes will be analyzed using the colorimetric kit and Seahorse XF to evaluate glycolytic and oxidative metabolism. Isolated monocytes CD14+ will be evaluating polarization of macrophages (M1 and M2) will be examined in the presence or absence of ¿2-adrenergic pathway activators. Data will be presented as mean and standard deviation, and the student's t-test or Mann-Whitney test will be used to compare groups. Adjusted comparisons will be performed using two-way analysis of covariance or generalized linear models and associations will be tested using linear regressions. (AU)