Changes in MMPs and inflammatory cells in experimental gingivitis

• Processo: 08/01528-0
• Modalidade de apoio: Auxílio à Pesquisa - Publicações científicas - Artigo
• Data de Início da vigência: 01 de abril de 2008
• Data de Término da vigência: 31 de março de 2009
• Área do conhecimento: Ciências Biológicas - Morfologia - Citologia e Biologia Celular
• Pesquisador responsável: Dagmar Ruth Stach - Machado
• Beneficiário: Dagmar Ruth Stach - Machado
• Instituição Sede: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brasil
• Assunto(s): Doenças periodontais Gengivite Metaloproteinases da matriz Publicações de divulgação científica Artigo científico
• Palavra(s)-Chave do Pesquisador: doença periodontal | gengivites | metaloproteinases de matriz | Inflamação

Resumo

In periodontal disease, extensive disorganization of the extracellular matrix promotes the loss of adhesion between the teeth and periodontium. A previous study suggested a reduction in the area occupied by collagen in the gingiva, during the first week of periodontal disease induction, however, the remaining fibers were more compact and thicker. Therefore, it was decided to investigate which of the MMP-2, -9, -14 and RECK, an MMP inhibitor, were involved in these modifications taking place in early gingivitis induced by ligature. The results of gene expression analysis indicated no changes for RECK. MMP-14 showed a reduction at 7 days of inflammation, and there was an immediate increase in MMP-2 gene expression and enzymatic activity, apparently by the stimulation of resident cells such as fibroblasts. A peak of MMP-9 expression 5 days after ligature followed after the peak of enzymatic activity found two days earlier. This pattern was consistent with the kinetics of macrophage and neutrophil recruitment. Immunohistochemistry suggested that MMP-9 was produced by both resident and inflammatory cells. Based on this evidence, it is suggested that extracellular matrix remodeling is related to MMP-2 and -9 production and activation. This allowed us to conclude that the host inflammatory response represents a significant factor for the advance of periodontal diseases. (AU)