Neuropathogenesis of Oropouche virus in a mouse model

• Processo: 13/50140-3
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Data de Início da vigência: 01 de junho de 2013
• Data de Término da vigência: 30 de novembro de 2015
• Área do conhecimento: Ciências Biológicas - Microbiologia - Biologia e Fisiologia dos Microorganismos
• Acordo de Cooperação: University of Michigan
• Pesquisador responsável: Eurico de Arruda Neto
• Beneficiário: Eurico de Arruda Neto
• Pesquisador Responsável no exterior: Katherine Spindler
• Instituição Parceira no exterior: University of Michigan, Estados Unidos
• Instituição Sede: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brasil
• Assunto(s): Arbovirus Infecções por Bunyaviridae Orthobunyavirus Barreira hematoencefálica Apoptose Sistema nervoso central
• Palavra(s)-Chave do Pesquisador: Apoptosis | Blood Brain Barrier | Mouse Model | Neuropathogenesis | Oropouche Virus

Resumo

Oropouche virus (OROV) causes a debilitating febrile disease second only to dengue fever among arboviral fevers in Brazil; there have been more than 30 epidemics since 1960. Bunyaviruses such as OROV are emerging tropical pathogens of significant concern, especially due to increased deforestation, global climate change, and international traveI. There is an urgent need for use of animal models of OROV infection to increase our knowledge about OROV pathogenesis, enabling development of therapeutic approaches for OROV. We have recently developed an OROV animal model in mice, in which central nervous system (CNS) disease develops that has similarities to OROV aseptic meningitis in people. We will determine how OROV infects the CNS, and whether it induces specific damage to the blood-brain barrier, including apoptosis. We will extend the mouse system to other strains and ages of mice to model the classic febrile form of disease. (AU)