EMBO Global Exchange Lecture Course on Structural and Biophysical Methods for Biological Macromolecules in Solution 2014 / São Paulo - Brazil

• Processo: 13/17943-5
• Modalidade de apoio: Auxílio Organização - Reunião Científica
• Data de Início da vigência: 19 de janeiro de 2014
• Data de Término da vigência: 26 de janeiro de 2014
• Área do conhecimento: Ciências Biológicas - Biofísica - Biofísica Molecular
• Pesquisador responsável: Cristiano Luis Pinto de Oliveira
• Beneficiário: Cristiano Luis Pinto de Oliveira
• Instituição Sede: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brasil
• Assunto(s): Calorimetria Ressonância magnética nuclear Proteínas
• Palavra(s)-Chave do Pesquisador: Calorimetry | macromolecules | Nmr | protein | Sas | Structure | Estrutura de Macromoléculas em Solução

Resumo

Structural methods applicable to solutions offer the unique opportunity to carry out structural and functional studies on biological macromolecules under virtually identical conditions. Fully exploiting this opportunity allows one to extract the maximum amount of biologically meaningful information and to relate the structural results to the function of the system. The main objective of the Course is to teach the Brazilian PhD students and postdocs from all areas of biology the methods applicable to study biological macromolecules in solution. We aim at a comprehensive coverage of the field including the major structural and biophysical techniques employed for the characterization of high and low resolution structure and structural transitions, macromolecular complex formation, protein folding and stability, protein-protein and protein-ligand interactions and enzymatic mechanisms. The Course will include lectures on small-angle X-ray and neutron scattering (SAXS/SANS), nuclear magnetic resonance (NMR), static and dynamic light scattering (SLS/DLS), analytical ultracentrifugation (AUC), differential and isothermal calorimetry (DSC/ITC) and spectroscopic approaches. Bioinformatic tools to analyze protein-protein interactions will also be considered, and the joint use of the solution characterization methods with the major non-solution structural techniques, macromolecular crystallography (MX), electron microscopy (EM), mass spectrometry (MS) and with the in situ methods will be covered. Special attention will be paid to interdisciplinary approaches, where the synergistic use of complementary techniques leads to a comprehensive description of macromolecular systems. It is proposed a 8-days course where these topics will covered in a intense an interactive way.Event Website:http://events.embo.org/14-macromolecule/ (AU)