Raman ratios on the repair of grafted surgical bone defects irradiated or not with laser (lambda 780 nm) or LED (lambda 850 nm)

Autor(es)

Pinheiro, Antonio Luiz B. ^{[1, 2, 3]} ; Soares, Luiz Guilherme P. ^[1] ; Marques, Aparecida Maria C. ^{[1, 2]} ; Aciole, Jouber Mateus S. ^[1] ; de Souza, Renato Aparecido ^[4] ; Silveira, Jr., Landulfo ^[3]

Afiliação do(s) autor(es)

^[1] Fed Univ Bahia UFBA, Sch Dent, Ctr Biophoton, BR-40110150 Salvador, BA - Brazil

^[2] Univ Sao Paulo, Natl Inst Opt & Photon, Phys Inst Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[3] Univ Camilo Castelo Branco UNICASTELO, Inst Biomed Engn, Sao Jose Dos Campos, SP - Brazil

^[4] Inst Fed Educ Ciencia & Tecnol Sul Minas Gerais I, Grp Estudos & Pesquisa Ciencias Saude, BR-37890000 Muzambinho, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 138, p. 146-154, SEP 5 2014.
• Citações Web of Science: 10

Resumo

This work aimed to assess biochemical changes associated to mineralization and remodeling of bone defects filled with Hydroxyapatite + Beta-Beta-tricalcium phosphate irradiated or not with 2 light sources. Ratios of intensities, band position and bandwidth of selected Raman peaks of collagen and apatites were used. Sixty male Wistar rats were divided into 6 groups subdivided into 2 subgroups (15th and 30th days). A standard surgical defect was created on one femur of each animal. In 3 groups the defects were filled with blood clot (Clot, Clot + Laser and Clot + LED groups) and in the remaining 3 groups the defects were filled with biomaterial (Biomaterial, Biomaterial + Laser and Biomaterial + LED groups). When indicated, the defects were irradiated with either Laser (lambda 780 nm, 70 mW, Phi similar to 0.4 cm(2)) or LED (lambda 850 +/- 10 rim, 150 mW, Phi similar to 0.5 cm(2)), 20 J/cm(2) each session, at 48 h intervals/2 weeks (140 J/cm(2) treatment). Following sacrifice, bone fragments were analyzed by Raman spectroscopy. Statistical analysis (ANOVA General Linear Model, p < 0.05) showed that both grafting and time were the variables that presented significance for the ratios of similar to 1660/similar to 1670 cm(-1) (collagen maturation), similar to 1077/similar to 854 cm(-1) (mineralization), similar to 1077/similar to 1070 cm(-1) (carbonate substitution) and the position of the similar to 960 cm(-1) (bone maturation). At 30th day, the ratios indicated an increased deposition of immature collagen for both Clot and Biomaterial groups. Biomaterial group showed increased collagen maturation. Only collagen deposition was significantly dependent upon irradiation independently of the light source, being the amount of collagen I increased in the Clot group at the end of the experimental time. On the other hand, collagen I deposition was reduced in biomaterial irradiated groups. Raman ratios of selected protein matrix and phosphate and carbonate HA indicated that the use of biphasic synthetic micro-granular HA + Beta-TCP graft improved the repair of bone defects, associated or not with Laser or LED light, because of the increasing deposition of HA. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/01788-5 - Espectroscopia Raman dispersiva utilizando fibras ópticas "Raman probe" aplicada ao diagnóstico de neoplasias na pele e próstata
• Beneficiário: Landulfo Silveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular