First detection of Leishmania infantum DNA within the brain of naturally infected dogs

Autor(es)

Grano, Fernanda G. ^[1] ; Melo, Guilherme D. ^[1] ; Belinchon-Lorenzo, Silvia ^[2] ; Gomez-Nieto, Luis C. ^[2] ; Machado, Gisele F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Coll Vet Med, Lab Appl Pathol LApap, Aracatuba, SP - Brazil

^[2] Univ Extremadura, Fac Vet, Parasitol Unit, LeishmanCeres Lab GLP Compliance Certified, Caceres - Spain

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 204, n. 3-4, p. 376-380, AUG 29 2014.
• Citações Web of Science: 7

Resumo

Visceral leishmaniasis is an anthropozoonosis caused by the protozoan Leishmania infantum (L. chagasi). In dogs, the disease presents with systemic manifestations, including neurological disorders. There are rare reports of the presence of the parasite in the central nervous system of infected dogs, and some evidences of inflammatory lesions and the breakdown of cerebral barriers have been described. The aim of this study was to investigate the presence of L infantum DNA in five specific areas of the brains of 20 naturally infected dogs by real-time PCR. For the first time, the presence of parasite DNA was detected and quantified in the brains of naturally infected dogs, in all evaluated regions. These data provide strong evidence of the presence of the Leishmania parasite in the nervous milieu and contribute to a new perspective of the pathogenesis of visceral leishmaniasis. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/03504-4 - Avaliação das subpopulações de linfócitos T em sangue e liquor de cães com leishmaniose visceral: correlação com lesões encefálicas
• Beneficiário: Fernanda Grecco Grano
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/10415-0 - Neuropatogenia da leishmaniose visceral canina: investigação de mediadores inflamatórios
• Beneficiário: Guilherme Dias de Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/21129-9 - Análise por PCR em tempo real para pesquisa de Leishmania chagasi no encéfalo de cães naturalmente infectados
• Beneficiário: Fernanda Grecco Grano
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado