Low-level laser therapy for osteonecrotic lesions: effects on osteoblasts treated with zoledronic acid

Autor(es)

Basso, Fernanda Goncalves ^[1] ; Silveira Turrioni, Ana Paula ^[1] ; Soares, Diana Gabiela ^[1] ; Bagnato, Vanderlei Salvador ^[2] ; Hebling, Josimeri ^[1] ; de Souza Costa, Carlos Alberto ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Araraquara Sch Dent, BR-14801903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Inst Phys, BR-13566590 Sao Carlos, SP - Brazil

^[3] Univ Estadual Paulista, Fac Odontol Araraquara, Dept Fisiol & Patol, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SUPPORTIVE CARE IN CANCER; v. 22, n. 10, p. 2741-2748, OCT 2014.
• Citações Web of Science: 5

Resumo

Clinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated. Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 mu M) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 +/- 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm(2), three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated. LLLT at 0.5 J/cm(2) increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm(2) but did not improve any other cell activity assessed. LLLT showed limited effects on bisphosphonate-treated osteoblasts. (AU)

• Processo FAPESP: 13/05879-0 - Efeito da laserterapia de baixa potência e fatores de crescimento sobre a atividade de fibroblastos de gengiva obtidos de pacientes jovens e idosos
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17947-8 - Efeito do laser de baixa intensidade sobre a expressão de citocinas inflamatórias na mucosite oral
• Beneficiário: Fernanda Gonçalves Basso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado