Burkholderia sp SCMS54 Triggers a Global Stress Defense in Tomato Enhancing Cadmium Tolerance

Autor(es)

Dourado, Manuella N. ^[1] ; Souza, Lucas A. ^{[2, 1]} ; Martins, Paula F. ^[1] ; Peters, Leila P. ^[1] ; Piotto, Fernando A. ^[1] ; Azevedo, Ricardo A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13400970 Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: WATER AIR AND SOIL POLLUTION; v. 225, n. 10 OCT 2014.
• Citações Web of Science: 12

Resumo

Cadmium is a toxic element for living organisms. This metal causes different damages to the cell, generating oxidative stress. In order to elucidate cadmium tolerance mechanism and increase tomato plant tolerance by inoculating a Cd-tolerant Burkholderia strain, we analyzed malondialdehyde, hydrogen peroxide content, and the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), and glutathione S-transferase of two strains, one isolated from a soil contaminated with Cd (strain SCMS54) and the other from a soil without Cd (strain SNMS32). Strains SNMS32 and SCMS54 exhibited different SOD, CAT, and GR isoenzyme profiles in non-denaturing polyacrylamide gel electrophoresis analysis, with strain SCMS54 exhibiting an extra isoenzyme for all enzymes (Mn-SOD, CAT I, and GR IV, respectively). Despite accumulating more Cd, strain SCMS54 did not increase peroxide hydrogen and presented a fast antioxidant response (increasing SOD and CAT after 5 h of Cd exposure). In this way, strain SCMS54 exhibited a higher metabolic diversity and plasticity when compared to strain SNMS32, so it was selected for Cd-Burkholderia-tomato interaction studies. Inoculated tomato plants in the presence of Cd grew more than non-inoculated plants with Cd indicating that the SCMS54 increased tomato Cd tolerance. It appears that the strain isolated from Cd-contaminated soil (SCMS54) triggers a global stress response in tomato increasing plant tolerance, which may enable plants to be cultivated in Cd-contaminated soils. (AU)

• Processo FAPESP: 09/54676-0 - Estresse oxidativo induzido por metais: novas abordagens
• Beneficiário: Ricardo Antunes de Azevedo
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/50368-9 - Efeito cádmio em bactérias isoladas de solo contaminado
• Beneficiário: Manuella Nóbrega Dourado Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado