Tropical Plant Extracts as Sources of Grain-Protectant Compounds Against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae)

Autor(es)

Ribeiro, L. P. ^[1] ; Vendramim, J. D. ^[1] ; Andrade, M. S. ^[2] ; Bicalho, K. U. ^[2] ; Silva, M. F. G. F. ^[2] ; Vieira, P. C. ^[2] ; Fernandes, J. B. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz deQueiroz, Dept Entomol & Acarol, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Quim, BR-13560 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neotropical Entomology; v. 43, n. 5, p. 470-482, OCT 2014.
• Citações Web of Science: 13

Resumo

The toxicity of organic plant extracts to Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) was assessed for three tropical plant species: branches, leaves, and seeds of Annona montana (Annonaceae), branches of Aristolochia paulistana (Aristolochiaceae), and leaves and branches of Casearia sylvestris (Salicaceae). The screening assay resulted that the extracts of A. montana seeds obtained with hexane (LC50 = 534.75 mg kg(-1) and LT50 = 6.10 days) and with dichloromethane (LC50 = 424.67 mg kg(-1) and LT50 = 5.03 days) were the most promising treatments, followed by the extract prepared from A. montana leaves with hexane (LC50 = 837.70 mg kg(-1) and LT50 = 4.90 days). Moreover, extracts (at 1,500 mg kg(-1)) prepared from C. sylvestris branches with dichloromethane and A. paulistana with hexane caused significant mortality (37% and 41.5%, respectively) beyond sublethal effects on S. zeamais. Therefore, based on the biological assays, extraction yield, and evaluation of the chromatographic profile of the crude extracts by TLC, the hexane extract of A. montana seeds was selected and fractioned using liquid-liquid partitioning. The hydroalcoholic fraction caused mortality of 55.5%, significantly superior to dichloromethane fraction, which caused 35.5% of mortality. Chemical analyses (H-1 NMR, HPLC, and TLC) were performed, and the results showed the presence of alkaloids and acetogenins in both active fractions, which have been associated with bioactivity. Therefore, extracts prepared from A. montana seeds (especially) is an interesting source of new compounds with promising grain-protectant properties. (AU)

• Processo FAPESP: 10/52638-0 - Prospeccao biomonitorada de substancias entomotoxicas em anonaceas neotropicais, utilizando sitophilus zeamais mots. (coleoptera: curculionidae) como bioindicador.
• Beneficiário: Leandro Do Prado Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/01847-1 - Obtenção de inseticida e fungicida contra formigas cortadeiras e seu fungo simbionte em Myracrodruon urundeuva e de complexos de coordenação com metais de compostos ativos para controle desta praga
• Beneficiário: André Lúcio Franceschini Sarria
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto