Evaluation of the application of enemas containing sucralfate in tissue content of neutral and acid mucins in experimental model of diversion colitis

Autor(es)

Chaim, Felipe Mendona ^[1] ; Sato, Daniela Tiemi ^[2] ; Rodrigues, Murilo Rocha ^[2] ; Dias, Alice Moreira ^[2] ; Silveira Junior, Paulo Pedroso ^[2] ; Pereira, Jose Aires ^[3] ; Real Martinez, Carlos Augusto ^{[4, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Postgraduate Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil

^[2] Univ Sao Francisco, Sch Med, BR-12900000 Braganca Paulista, SP - Brazil

^[3] Univ Sao Francisco, Postgrad Program Hlth Sci, BR-12900000 Braganca Paulista, SP - Brazil

^[4] Univ Estadual Campinas, Dept Surg, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 29, n. 9, p. 544-552, SEP 2014.
• Citações Web of Science: 6

Resumo

PURPOSE: To evaluate the effects of sucralfate on tissue content of neutral and acids mucins in rats with diversion colitis. METHODS: Thirty-six rats were submitted to a proximal right colostomy and a distal mucous fistula. They were divided into two groups according to sacrifice to be performed two or four weeks after intervention. Each group was divided into three subgroups according daily application of enemas containing saline, sucralfate at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis and neutral and acid mucins by Periodic Acid Schiff and Alcian Blue techniques, respectively. The contents of mucins were quantified by computer-assisted image analysis. Student's t paired and ANOVA test were used to compare the contents of both types of mucins among groups, and to verify the variance with time, establishing level of signification of 5% for both (p<0.05). RESULTS: Enemas containing sucralfate improves the inflammation and increases the tissue contents of neutral and acid mucins. The content of neutral mucins does not change with the time or concentration of sucralfate used, while acid mucins increases with concentration and time of intervention. CONCLUSIONS: Sucralfate enemas improve the inflammatory process and increase the tissue content of neutral and acid mucins in colon without fecal stream. (AU)

• Processo FAPESP: 10/12492-7 - Avaliação dos efeitos do ácido 5-aminosalicilico, N-acetilcisteína, sucralfato, curcumina e extrato aquoso de Ilex paraguariensis no conteúdo e padrão de expressão das proteínas das junções intercelulares: estudo em ratos
• Beneficiário: Carlos Augusto Real Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular