Light-triggered and cysteine-mediated nitric oxide release from a biodegradable starch-based film

Autor(es)

Roveda, Jr., Antonio Carlos ^[1] ; Aguiar, Helena de Fazio ^[2] ; Miranda, Katrina M. ^[3] ; Tadini, Carmen Cecilia ^[2] ; Franco, Douglas Wagner ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Escola Politecn, Dept Chem Engn, BR-05508010 Sao Paulo - Brazil

^[3] Univ Arizona, Dept Chem & Biochem, Tucson, AZ 85721 - USA

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS CHEMISTRY B; v. 2, n. 41, p. 7232-7242, 2014.
• Citações Web of Science: 7

Resumo

A new nitric oxide-releasing material produced with cassava starch is described. The ruthenium nitrosyl complex trans-{[}Ru(NH3)(4)(isn)NO](BF4)(3) (RuNOisn; isn = isonicotinamide) is able to release NO upon either photolysis or chemical reduction. Impregnating this complex under mild conditions into cassava starch (CS) films produced a NO-delivery platform (CSx-RuNOisn). Spectroscopic analysis of CSx-RuNOisn indicates that the coordination sphere of RuNOisn remains intact during film production. Exposure of CSx-RuNOisn to long wave UV-light (lambda(irr) = 355 nm) leads to NO release and formation of the paramagnetic photoproduct trans-{[}RuIII(NH3)(4)isn(H2O)](3+) in the CS film. Reaction of this aquaruthenium(III) complex with aqueous nitrite regenerates RuNOisn in the film. Delivery of NO upon photolysis of CSx-RuNOisn was verified by trapping with oxymyoglobin. Moreover, NO release upon chemical reduction was carried out using L-cysteine as a reductant. Cysteine-mediated NO delivery from CSx-RuNOisn persisted for more than 7 h, during which physiologically relevant NO concentrations were liberated. These results suggest that CSx-RuNOisn is a promising candidate for use in biological applications. (AU)

• Processo FAPESP: 12/23651-4 - Nitrosilo e nitroxila como ligantes em Ru(II): síntese, reatividade, cinética e termodinâmica
• Beneficiário: Douglas Wagner Franco
• Modalidade de apoio: Auxílio à Pesquisa - Regular