Cryotolerance and global gene-expression patterns of Bos taurus indicus and Bos taurus taurus in vitro- and in vivo-produced blastocysts

Autor(es)

Sudano, Mateus J. ^[1] ; Caixeta, Ester S. ^[2] ; Paschoal, Daniela M. ^[1] ; Martins, Jr., Alicio ^[3] ; Machado, Rui ^[4] ; Buratini, Jose ^[5] ; Landim-Alvarenga, Fernanda D. C. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Jose Rosario Vellano UNIFENAS, Dept Anim Reprod, BR-37130000 Alfenas, MG - Brazil

^[3] Sao Paulo State Univ, Sch Vet Med, Dept Clin Surg & Anim Reprod, BR-16050680 Aracatuba, SP - Brazil

^[4] Embrapa Southeast Cattle, BR-13560970 Sao Carlos, SP - Brazil

^[5] Sao Paulo State Univ, Dept Physiol, BR-18618970 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 26, n. 8, p. 1129-1141, 2014.
• Citações Web of Science: 10

Resumo

In a 2x2 factorial experimental design, embryo development, cryotolerance and global gene expression of Nellore (Bos taurus indicus) and Simmental (Bos taurus taurus) blastocysts produced in vitro (IVP) and in vivo (multiple ovulation derived embryo, MODE) were assessed. Blastocyst production was higher in Nellore than in Simmental (47.7 +/- 2.0% vs 27.0 +/- 2.0%) cows. The total numbers of ova or embryos recovered (5.5 +/- 0.9 vs 3.7 +/- 0.8) and transferable embryos (3.8 +/- 1.0 vs 2.3 +/- 0.8) per cow were not different between breeds. Simmental and MODE (34.6% and 38.5%, n=75 and 70) blastocysts had higher survival rates after cryopreservation compared with Nellore and IVP (20.2% and 18.1%, n=89 and 94) embryos, respectively. Differences between transcriptomes were addressed by principal-component analysis, which indicated that gene expression was affected by subspecies (158 genes), origin (532 genes) and interaction between both subspecies and origin (53 genes). Several functional processes and pathways relevant to lipid metabolism and embryo viability involving differentially expressed genes were identified. The lipid metabolism-related genes were upregulated in Simmental (AUH and ELOVL6) and IVP (ACSL3 and ACSL6) blastocysts. The expression profiles of genes related to mitochondrial metabolism (ATP5B), oxidative stress (GPX4), apoptosis (DAD1, DAP, PRDX2), heat shock (HSPA5), pregnancy (IFNT2, PAG2) and cell differentiation (KRT18) varied between experimental groups. (AU)

• Processo FAPESP: 10/09922-0 - Comparação do padrão da expressão gênica global de embriões Bos taurus indicus e Bos taurus taurus produzidos in vitro
• Beneficiário: Fernanda da Cruz Landim
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/02333-4 - Comparação do padrão da expressão gênica global de embriões Bos taurus indicus e Bos taurus taurus produzidos in vitro e in vivo
• Beneficiário: Mateus José Sudano
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 09/54513-3 - Comparação do padrão da expressão gênica de embriões Bos taurus indicus e Bos taurus taurus produzidos in vitro e in vivo, frescos e criopreservados
• Beneficiário: Mateus José Sudano
• Modalidade de apoio: Bolsas no Brasil - Doutorado