Graphene and carbon nanotube nanocomposite for gene transfection

Autor(es)

Hollanda, L. M. ^[1] ; Lobo, A. O. ^[2] ; Lancellotti, M. ^[1] ; Berni, E. ^[3] ; Corat, E. J. ^[4] ; Zanin, H. ^[4]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, Biotechnol Lab, BR-13083862 Campinas, SP - Brazil

^[2] UNIVAP, Inst Res & Dev, Lab Biomed Nanotechnol, BR-12244000 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Estadual Campinas, Inst Chem, Dept Phys Chem, Biol Chem Lab, BR-13083970 Campinas, SP - Brazil

^[4] INPE, Associated Lab Sensors & Mat, BR-12227010 Sao Jose Dos Campos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 39, p. 288-298, JUN 1 2014.
• Citações Web of Science: 40

Resumo

Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also known to attach oxygen content groups on the MWCNT surfaces, changing their hydrophobicity. The nanocomposite was characterised by high resolution scanning electron microscopy; energy-dispersive X-ray, Fourier transform infrared. and Raman spectroscopies, as well as zeta potential and particle size analyses using dynamic light scattering. BET adsorption isotherms showed the GCN to have an effective surface area of 38.5 m(2)/g. The GCN and pIRES plasmid conjugated with the GFP gene, forming it-stacking when dispersed in water by magnetic stirring, resulting in a helical wrap. The measured zeta potential confirmed that the plasmid was connected to the nanocomposite. The NIH-3T3 and NG97 cell lines could phagocytize this wrap. The gene transfection was characterised by fluorescent protein produced in the cells and pictured by fluorescent microscopy. Before application, we studied GCN cell viability in NIH-3T3 and NG97 line cells using both MTT and Neutral Red uptake assays. Our results suggest that GCN has moderate stability behaviour as colloid solution and has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity and good transfection efficiency. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/17195-3 - Nanopartículas mesoporosas de sílica como estratégia na terapeutica antitumoral
• Beneficiário: Luciana Maria de Hollanda
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/17877-7 - Desenvolvimento de novos scaffolds poliméricos por eletrofiação com incorporação de nanotubos alinhados e nanohidroxiapatita para regeneração óssea
• Beneficiário: Anderson de Oliveira Lobo
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores