| Texto completo | |
| Autor(es): |
Campos, C. D. M.
[1, 2]
;
Park, J. K.
[2]
;
Neuzil, P.
[2]
;
da Silva, J. A. F.
[1, 3]
;
Manz, A.
[2]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Chem, BR-13083861 Campinas, SP - Brazil
[2] KIST Europe, D-66123 Saarbrucken - Germany
[3] INCTBio, Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | RSC ADVANCES; v. 4, n. 90, p. 49485-49490, 2014. |
| Citações Web of Science: | 5 |
| Resumo | |
In this paper we report a method of continuous electroextraction of amino acids as model samples using a caseinate-poly(ethylene-glycol) - PEG - two phase system in a microchip able to separate compounds based on their differences in electrophoretic mobility and solvent affinity. Fundamentally, the phase boundary replaces a physical membrane, suppressing the diffusion. When external potential is applied, the molecules selectively cross this barrier. The selectivity of the amino acids extraction is the result of their electrochemical properties and applied voltage. We applied this method to amino acids extraction and the results suggest the possibility of high levels of purification by controlling the electric field strength across the liquid barrier. It is a promising method for complex sample separation as well as for the purification of individual compounds. (AU) | |
| Processo FAPESP: | 13/06625-2 - Desenvolvimento de microdispositivos enzimáticos para avaliação de glutamato em matrizes complexas |
| Beneficiário: | Camila Dalben Madeira Campos |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 11/02477-3 - Desenvolvimento de um Lab-on-a-chip para análise de glutamato em alimentos |
| Beneficiário: | Camila Dalben Madeira Campos |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |