Developmental toxicity, acute toxicity and mutagenicity testing in freshwater snails Biomphalaria glabrata (Mollusca: Gastropoda) exposed to chromium and water samples

Autor(es)

Tallarico, Lenita de Freitas ^{[1, 2]} ; Borrely, Sueli Ivone ^[1] ; Hamada, Natalia ^[1] ; Grazeffe, Vanessa Siqueira ^[2] ; Ohlweiler, Fernanda Pires ^[3] ; Okazaki, Kayo ^[1] ; Granatelli, Amanda Tosatte ^[2] ; Pereira, Ivana Wuo ^[4] ; de Braganca Pereira, Carlos Alberto ^[5] ; Nakano, Eliana ^[2]

Afiliação do(s) autor(es)

^[1] IPEN CNEN SP, BR-05508000 Sao Paulo - Brazil

^[2] Inst Butantan, Lab Parasitol Malacol, BR-05503900 Sao Paulo - Brazil

^[3] Superintendencia Controle Endemias Estado Sao Paul, Lab Malacol, BR-05408003 Sao Paulo - Brazil

^[4] Companhia Saneamento Basico Estado Sao Paulo, BR-08680000 Suzano, SP - Brazil

^[5] Univ Sao Paulo, Inst Matemat & Estat, Dept Estat, BR-05008090 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 110, p. 208-215, DEC 2014.
• Citações Web of Science: 13

Resumo

A protocol combining acute toxicity, developmental toxicity and mutagenicity analysis in freshwater snail Biomphalaria glabrata for application in ecotoxicological studies is described. For acute toxicity testing, LC50 and EC50 values were determined; dominant lethal mutations induction was the endpoint for mutagenicity analysis. Reference toxicant potassium dichromate (K2Cr2O7) was used to characterize B. glabrata sensitivity for toxicity and cyclophosphamide to mutagenicity testing purposes. Compared to other relevant freshwater species, B. glabrata showed high sensitivity: the lowest EC50 value was obtained with embryos at veliger stage (5.76 mg/L). To assess the model applicability for environmental studies, influent and effluent water samples from a wastewater treatment plant were evaluated. Gastropod sensitivity was assessed in comparison to the standardized bioassay with Daphnia similis exposed to the same water samples. Sampling sites identified as toxic to daphnids were also detected by snails, showing a qualitatively similar sensitivity suggesting that B. glabrata is a suitable test species for freshwater monitoring. Holding procedures and protocols implemented for toxicity and developmental bioassays showed to be in compliance with international standards for intra-laboratory precision. Thereby, we are proposing this system for application in ecotoxicological studies. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 05/60237-8 - Aplicacao de ensaios biologicos na avaliacao da qualidade da agua do rio tiete.
• Beneficiário: Eliana Nakano
• Modalidade de apoio: Auxílio à Pesquisa - Regular