Cytomegalovirus Infection in Inflammatory Bowel Disease Is Not Associated with Worsening of Intestinal Inflammatory Activity

Autor(es):Mostrar menos -

do Carmo, Alexandre Medeiros ^[1] ; Santos, Fabiana Maria ^[1] ; Ortiz-Agostinho, Carmen Lucia ^[1] ; Nishitokukado, Ieda ^[1] ; Frota, Cintia S. ^[1] ; Gomes, Flavia Ubeda ^[1] ; de Arruda Leite, Andre Zonetti ^[1] ; Pannuti, Claudio Sergio ^{[2, 3]} ; Vilas Boas, Lucy Santos ^{[4, 5]} ; Teixeira, Magaly Gemio ^[6] ; Sipahi, Aytan Miranda ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin, Dept Gastroenterol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Inst Trop Med, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Dept Doencas Infecciosas & Parasitarias LIM HC, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med LIM HC, Inst Trop Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med LIM HC, Hosp Clin, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Fac Med, Hosp Clin, Serv Cirurgia Colon Reto & Anus, Dept Cirurgia, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 9, n. 11 NOV 11 2014.
• Citações Web of Science: 11

Resumo

Background: Cytomegalovirus is highly prevalent virus and usually occurs in immunocompromised patients. The pathophysiology and treatment of inflammatory bowel disease often induce a state of immunosuppression. Because this, there are still doubts and controversies about the relationship between inflammatory bowel disease and cytomegalovirus. Aim: Evaluate the frequency of cytomegalovirus in patients with inflammatory bowel disease and identify correlations. Methods: Patients with inflammatory bowel disease underwent an interview, review of records and collection of blood and fecal samples. The search for cytomegalovirus was performed by IgG and IgM blood serology, by real-time PCR in the blood and by qualitative PCR in feces. Results were correlated with red blood cell levels, C-reactive protein levels, erythrocyte sedimentation rates and fecal calprotectin levels for each patient. Results: Among the 400 eligible patients, 249 had Crohn's disease, and 151 had ulcerative colitis. In the group of Crohn's disease, 67 of the patients had moderate or severe disease, but 126 patients presented with active disease, based on the evaluation of the fecal calprotectin. In patients with ulcerative colitis, only 21 patients had moderate disease, but 76 patients presented with active disease, based on the evaluation of the fecal calprotectin. A large majority of patients had positive CMV IgG. Overall, 10 patients had positive CMV IgM, and 9 patients had a positive qualitative detection of CMV DNA by PCR in the feces. All 400 patients returned negative results after the quantitative detection of CMV DNA in blood by real-time PCR. Analyzing the 19 patients with active infections, we only found that such an association occurred with the use of combined therapy (anti-TNF-alpha + azathioprine) Conclusion: The findings show that latent cytomegalovirus infections are frequent and active cytomegalovirus infection is rare. We did not find any association between an active infection of CMV and inflammatory bowel disease activity. (AU)

• Processo FAPESP: 10/18275-8 - Doença inflamatória intestinal e citomegalovírus
• Beneficiário: Aytan Miranda Sipahi
• Modalidade de apoio: Auxílio à Pesquisa - Regular