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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Hippocampal expression of heat shock proteins in mesial temporal lobe epilepsy with psychiatric comorbidities and their relation to seizure outcome

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Autor(es):
Kandratavicius, Ludmyla [1, 2] ; Hallak, Jaime E. [1, 2, 3] ; Carlotti, Jr., Carlos G. [4] ; Assirati, Jr., Joao A. [4] ; Leite, Joao P. [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Ctr Interdisciplinary Res Appl Neurosci NAPNA, BR-14049900 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Neurosci & Behav, BR-14049900 Ribeirao Preto, SP - Brazil
[3] Natl Inst Sci & Technol Translat Med INCT TM CNPq, Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Surg, BR-14049900 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Epilepsia; v. 55, n. 11, p. 1834-1843, NOV 2014.
Citações Web of Science: 16
Resumo

ObjectiveBiologic substrates behind the close association between mesial temporal lobe epilepsy (MTLE) and psychiatric comorbidities are largely unknown. Heat shock protein 70 (HSP70) and HSP90 are ubiquitous molecular chaperones that play important roles in functions from cellular stress response to receptor trafficking control. There are controversial findings regarding HSP expression in epilepsy. Our goal was to examine HSP70 and HSP90 expression within the human hippocampal formation of MTLE patients with and without comorbid major depression and psychosis. In addition, we investigated the possible correlation between HSP expression and seizure outcome. MethodsMTLE hippocampi of subjects without psychiatric history, MTLE and major depression, and MTLE and interictal psychosis derived from epilepsy surgery and control necropsies were investigated for neuronal densities, HSP70 and HSP90 immunoreactive area. ResultsIncreased HSP expression in MTLE and decreased HSP expression in MTLE with psychosis cases were detailed. Patients taking fluoxetine showed increased HSP90 expression in CA1, and those taking haloperidol decreased HSP90 in the granular layer and subiculum. MTLE patients with complete seizure remission presented with decreased HSP70 expression in CA4 and subiculum and decreased HSP90 expression in the granular layer. SignificanceThe present results provide the first demonstration of HSP expression in human MTLE hippocampal formation with and without psychiatric comorbidities. Distinct HSP70 and HSP90 expression might explain some of the structural and synaptic alterations differentially regulated in MTLE with and without psychiatric comorbidities. Increased HSPs expression in key hippocampal subfields would reflect increased epileptogenicity and poorer outcome of epilepsy surgery. (AU)

Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
Beneficiário:João Pereira Leite
Linha de fomento: Auxílio à Pesquisa - Programa CINAPCE - Temático