| Texto completo | |
| Autor(es): |
Libardi, S. H.
[1]
;
Pindstrup, H.
[2]
;
Amigo, J. M.
[2]
;
Cardoso, D. R.
[1]
;
Skibsted, L. H.
[2]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Chem Inst Sao Carlos, BR-13560470 Sao Carlos, SP - Brazil
[2] Univ Copenhagen, Dept Food Sci, DK-1958 Frederiksberg C - Denmark
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | RSC ADVANCES; v. 4, n. 105, p. 60953-60958, 2014. |
| Citações Web of Science: | 6 |
| Resumo | |
Reduction of the hypervalent meat pigment ferrylmyoglobin, MbFe(IV)=O, by cysteine is enhanced by acid due to protonation of ferrylmyoglobin to yield sulfmyoglobin as the main product, while at alkaline conditions, the rate decreases with the cysteine dianion as the reactant forming oxymyoglobin, MbFe(II) O-2. The second-order rate constant for cysteine reacting with protonated ferrylmyoglobin is 5.1 +/- 0.4 L mol(-1) s(-1) at 25 degrees C in 0.16 M aqueous sodium chloride and for the cysteine dianion reacting with ferrylmyoglobin 0.31 +/- 0.15 L mol(-1) s(-1). For pH = 7.4 the activation parameters for sulfmyoglobin formation is Delta H double dagger = 75 +/- 2 kJ mol(-1) and Delta S double dagger = -250 +/- 7 J mol(-1) K-1 with similar values for homocysteine and glutathione. The difference in product is indicative of a shift from an electron-transfer/radical addition mechanism at low pH as in the stomach to a two-step electron-transfer mechanism at higher pH as in the intestine, and is discussed in relation to protection against the formation of radicals by sulphurous compounds during the digestion of red meat. (AU) | |
| Processo FAPESP: | 11/51555-7 - Pão e carne para o futuro |
| Beneficiário: | Daniel Rodrigues Cardoso |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |