Methylene blue attenuates ischemia-reperfusion injury in lung transplantation

Autor(es)

Abreu, Marcus da Matta ^[1] ; Pazetti, Rogerio ^[1] ; de Almeida, Francine Maria ^[1] ; Correia, Aristides Tadeu ^[1] ; Parra, Edwin Roger ^[2] ; da Silva, Lais Pereira ^[1] ; Vieira, Rodolfo de Paula ^[3] ; Pego-Fernandes, Paulo Manuel ^[1] ; Jatene, Fabio Biscegli ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Fac Med, Heart Inst InCor, Dept Cardiopneumol, BR-05403900 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Dept Pathol, BR-05403900 Sao Paulo - Brazil

^[3] Nove Julho Univ UNINOVE, Dept Res, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF SURGICAL RESEARCH; v. 192, n. 2, p. 635-641, DEC 2014.
• Citações Web of Science: 12

Resumo

Background: Ischemia-reperfusion injury (IRI) is one of the principal obstacles for the lung transplantation (LTx) success. Several strategies have been adopted to minimize the effects of IRI in lungs, including ex vivo conditioning of the grafts and the use of antioxidant drugs, such as methylene blue (MB). We hypothesized that MB could minimize the effects of IRI in a LTx rodent model. Methods: Forty rats were divided into four groups (n = 10) according to treatment (saline solution or MB) and graft cold ischemic time (3 or 6 h). All animals underwent unilateral LTx. Recipients received 2 mL of saline or MB intraperitoneally before transplantation. After 2 h of reperfusion, arterial blood and exhaled nitric oxide samples were collected and bronchoalveolar lavage performed. Then animals were euthanized, and histopathology analysis as well as cell counts and cytokine levels measurements in bronchoalveolar lavage fluid were performed. Results: There was a significant decrease in exhaled nitric oxide, neutrophils, interleukin-6, and tumor necrosis factor-alpha in MB-treated animals. PaO2 and uric acid levels were higher in MB group. Conclusions: MB was able in attenuating IRI in this LTx model. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/50715-0 - O papel do azul de metileno na prevenção da lesão de isquemia-reperfusão em transplante pulmonar de ratos: estudo experimental
• Beneficiário: Fabio Biscegli Jatene
• Modalidade de apoio: Auxílio à Pesquisa - Regular