Flavonoid supplementation affects the expression of genes involved in cell wall formation and lignification metabolism and increases sugar content and saccharification in the fast-growing eucalyptus hybrid E-urophylla x E-grandis

Autor(es):Mostrar menos -

Lepikson-Neto, Jorge ^[1] ; Nascimento, Leandro C. ^[1] ; Salazar, Marcela M. ^[1] ; Camargo, Eduardo L. O. ^[1] ; Cairo, Joao P. F. ^[2] ; Teixeira, Paulo J. ^[1] ; Marques, Wesley L. ^[1] ; Squina, Fabio M. ^[2] ; Mieczkowski, Piotr ^[3] ; Deckmann, Ana C. ^[1] ; Pereira, Goncalo A. G. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Lab Genom & Expressao, Campinas, SP - Brazil

^[2] CTBE, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas, SP - Brazil

^[3] Univ N Carolina, Sch Med, Dept Genet, Chapel Hill, NC - USA

• Tipo de documento: Artigo Científico
• Fonte: BMC PLANT BIOLOGY; v. 14, NOV 19 2014.
• Citações Web of Science: 5

Resumo

Background: Eucalyptus species are the most widely planted hardwood species in the world and are renowned for their rapid growth and adaptability. In Brazil, one of the most widely grown Eucalyptus cultivars is the fast-growing Eucalyptus urophylla x Eucalyptus grandis hybrid. In a previous study, we described a chemical characterization of these hybrids when subjected to flavonoid supplementation on 2 distinct timetables, and our results revealed marked differences between the wood composition of the treated and untreated trees. Results: In this work, we report the transcriptional responses occurring in these trees that may be related to the observed chemical differences. Gene expression was analysed through mRNA-sequencing, and notably, compared to control trees, the treated trees display differential down-regulation of cell wall formation pathways such as phenylpropanoid metabolism as well as differential expression of genes involved in sucrose, starch and minor CHO metabolism and genes that play a role in several stress and environmental responses. We also performed enzymatic hydrolysis of wood samples from the different treatments, and the results indicated higher sugar contents and glucose yields in the flavonoid-treated plants. Conclusions: Our results further illustrate the potential use of flavonoids as a nutritional complement for modifying Eucalyptus wood, since, supplementation with flavonoids alters its chemical composition, gene expression and increases saccharification probably as part of a stress response. (AU)

• Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
• Beneficiário: Munir Salomao Skaf
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 12/22652-7 - O Eucalipto como fonte de açúcar para a produção de etanol.
• Beneficiário: Jorge Lepikson Neto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado