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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Flavonoid supplementation affects the expression of genes involved in cell wall formation and lignification metabolism and increases sugar content and saccharification in the fast-growing eucalyptus hybrid E-urophylla x E-grandis

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Lepikson-Neto, Jorge [1] ; Nascimento, Leandro C. [1] ; Salazar, Marcela M. [1] ; Camargo, Eduardo L. O. [1] ; Cairo, Joao P. F. [2] ; Teixeira, Paulo J. [1] ; Marques, Wesley L. [1] ; Squina, Fabio M. [2] ; Mieczkowski, Piotr [3] ; Deckmann, Ana C. [1] ; Pereira, Goncalo A. G. [1]
Total Authors: 11
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Lab Genom & Expressao, Campinas, SP - Brazil
[2] CTBE, Lab Nacl Ciencia & Tecnol Bioetanol, Campinas, SP - Brazil
[3] Univ N Carolina, Sch Med, Dept Genet, Chapel Hill, NC - USA
Total Affiliations: 3
Document type: Journal article
Source: BMC PLANT BIOLOGY; v. 14, NOV 19 2014.
Web of Science Citations: 5
Abstract

Background: Eucalyptus species are the most widely planted hardwood species in the world and are renowned for their rapid growth and adaptability. In Brazil, one of the most widely grown Eucalyptus cultivars is the fast-growing Eucalyptus urophylla x Eucalyptus grandis hybrid. In a previous study, we described a chemical characterization of these hybrids when subjected to flavonoid supplementation on 2 distinct timetables, and our results revealed marked differences between the wood composition of the treated and untreated trees. Results: In this work, we report the transcriptional responses occurring in these trees that may be related to the observed chemical differences. Gene expression was analysed through mRNA-sequencing, and notably, compared to control trees, the treated trees display differential down-regulation of cell wall formation pathways such as phenylpropanoid metabolism as well as differential expression of genes involved in sucrose, starch and minor CHO metabolism and genes that play a role in several stress and environmental responses. We also performed enzymatic hydrolysis of wood samples from the different treatments, and the results indicated higher sugar contents and glucose yields in the flavonoid-treated plants. Conclusions: Our results further illustrate the potential use of flavonoids as a nutritional complement for modifying Eucalyptus wood, since, supplementation with flavonoids alters its chemical composition, gene expression and increases saccharification probably as part of a stress response. (AU)

FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/22652-7 - Eucalyptus as sugar provider for second generation ethanol production
Grantee:Jorge Lepikson Neto
Support Opportunities: Scholarships in Brazil - Post-Doctoral