High-Resolution Transcript Profiling of the Atypical Biotrophic Interaction between Theobroma cacao and the Fungal Pathogen Moniliophthora perniciosa

Autor(es):Mostrar menos -

Pereira Lima Teixeira, Paulo Jose ^[1] ; de Toledo Thomazella, Daniela Paula ^[1] ; Reis, Osvaldo ^[1] ; Vital do Prado, Paula Favoretti ^[1] ; Scatolin do Rio, Maria Carolina ^[1] ; Fiorin, Gabriel Lorencini ^[1] ; Jose, Juliana ^[1] ; Lacerda Costa, Gustavo Gilson ^[1] ; Negri, Victor Augusti ^[1] ; Costa Mondego, Jorge Mauricio ^[2] ; Mieczkowski, Piotr ^[3] ; Guimaraes Pereira, Goncalo Amarante ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Lab Genom & Expressao, BR-13083970 Campinas, SP - Brazil

^[2] Inst Agron Estado Sao Paulo, Ctr Pesquisa & Desenvolvimento Recursos Genet Veg, BR-13001970 Campinas, SP - Brazil

^[3] Univ N Carolina, Sch Med, Dept Genet, Chapel Hill, NC 27599 - USA

• Tipo de documento: Artigo Científico
• Fonte: PLANT CELL; v. 26, n. 11, p. 4245-4269, NOV 2014.
• Citações Web of Science: 40

Resumo

Witches' broom disease (WBD), caused by the hemibiotrophic fungus Moniliophthora perniciosa, is one of the most devastating diseases of Theobroma cacao, the chocolate tree. In contrast to other hemibiotrophic interactions, the WBD biotrophic stage lasts for months and is responsible for the most distinctive symptoms of the disease, which comprise drastic morphological changes in the infected shoots. Here, we used the dual RNA-seq approach to simultaneously assess the transcriptomes of cacao and M. perniciosa during their peculiar biotrophic interaction. Infection with M. perniciosa triggers massive metabolic reprogramming in the diseased tissues. Although apparently vigorous, the infected shoots are energetically expensive structures characterized by the induction of ineffective defense responses and by a clear carbon deprivation signature. Remarkably, the infection culminates in the establishment of a senescence process in the host, which signals the end of the WBD biotrophic stage. We analyzed the pathogen's transcriptome in unprecedented detail and thereby characterized the fungal nutritional and infection strategies during WBD and identified putative virulence effectors. Interestingly, M. perniciosa biotrophic mycelia develop as long-termparasites that orchestrate changes in plant metabolism to increase the availability of soluble nutrients before plant death. Collectively, our results provide unique insight into an intriguing tropical disease and advance our understanding of the development of (hemi) biotrophic plant-pathogen interactions. (AU)

• Processo FAPESP: 12/09136-0 - Genômica comparativa de fungos do gênero Moniliophthora: entendendo a biologia e estratégias de infecção dos principais patógenos do cacaueiro
• Beneficiário: Daniela Paula de Toledo Thomazella
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/51018-1 - Construção de um atlas transcriptômico para o estudo da doença vassoura-de-bruxa do cacaueiro
• Beneficiário: Paulo José Pereira Lima Teixeira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 06/59843-3 - Histologia da interação planta patógeno em Theobroma cacao L. infectado por Crinipellis perniciosa (Stahel) Singer durante a doença vassoura de bruxa: aspectos estruturais, histoquímicos e imunocitoquímicos
• Beneficiário: Maria Carolina Scatolin Do Rio
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/50119-9 - Estudo integrado e comparativo de três doenças fúngicas do cacau: vassoura-de-bruxa, monilíase e mal do facão, visando à compreensão de mecanismos de patogenicidade para o desenvolvimento de estratégias de controle
• Beneficiário: Gonçalo Amarante Guimarães Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Temático