Surface engineering of silica nanoparticles for oral insulin delivery: Characterization and cell toxicity studies

Autor(es):Mostrar menos -

Andreani, Tatiana ^{[1, 2, 3]} ; Kiill, Charlene P. ^[4] ; de Souza, Ana Luiza R. ^[4] ; Fangueiro, Joana F. ^{[3, 5]} ; Fernandes, Lisete ^[6] ; Doktorovova, Slavomira ^[7] ; Santos, Dario L. ^{[1, 2]} ; Garcia, Maria L. ^[8] ; Gremiao, Maria Palmira D. ^[4] ; Souto, Eliana B. ^[9] ; Silva, Amelia M. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Tras Os Montes & Alto Douro, UTAD, Dept Biol & Environm, P-5001801 Quinta De Prados, Vila Real - Portugal

^[2] UTAD, CITAB, Ctr Res & Technol Agroenvironm & Biol Sci, Vila Real - Portugal

^[3] UFP, Fernando Pessoa Univ, P-4249004 Oporto - Portugal

^[4] Univ Estadual Paulista, Univ Estadual Paulista, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[5] UFP, Fernando Pessoa Univ, CEBIMED, Res Ctr Biomed, P-4249004 Oporto - Portugal

^[6] UTAD, Electron Microscopy Unit, Vila Real - Portugal

^[7] Univ Lisbon, Fac Pharm, Res Inst Med & Pharmaceut Sci iMed UL, P-1649003 Lisbon - Portugal

^[8] Univ Barcelona, Fac Pharm, Dept Phys Chem, E-08028 Barcelona - Spain

^[9] Univ Coimbra FFUC, Dept Pharmaceut Technol, Fac Pharm, Polo Ciencias Saude, P-3000548 Coimbra - Portugal

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 123, p. 916-923, NOV 1 2014.
• Citações Web of Science: 38

Resumo

The present work aimed at studying the interaction between insulin and SiNP surfaced with mucoadhesive polymers (chitosan, sodium alginate or polyethylene glycol) and the evaluation of their biocompatibility with HepG2 and Caco-2 cell lines, which mimic in vivo the target of insulin-loaded nanoparticles upon oral administration. Thus, a systematic physicochemical study of the surface-modified insulin-silica nanoparticles (Ins-SiNP) using mucoadhesive polymers has been described. The surfacing of nanoparticle involved the coating of silica nanoparticles (SiNP) with different mucoadhesive polymers, to achieve high contact between the systems and the gut mucosa to enhance the oral insulin bioavailability. SiNP were prepared by a modified Stoner method at room temperature via hydrolysis and condensation of tetraethyl orthosilicate (TEOS). Interaction between insulin and nanoparticles was assessed by differential scanning calorimetry (DSC), X-ray and Fourier-transform infrared (FTIR) studies. The high efficiency of nanoparticles' coating resulted in more stable system. FTIR spectra of insulin-loaded nanoparticles showed amide absorption bands which are characteristic of alpha-helix content. In general, all developed nanoparticles demonstrated high biocompatible, at the tested concentrations (50-500 mu g/mL), revealing no or low toxicity in the two human cancer cell lines (HepG2 and Caco-2). In conclusion, the developed insulin-loaded SiNP surfaced with mucoadhesive polymers demonstrated its added value for oral administration of proteins. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/10174-3 - Avaliação de nanopartículas de quitosana para liberação de inibidores de integrinas potencialmente ativos em glioblastomas
• Beneficiário: Charlene Priscila Kiill
• Modalidade de apoio: Bolsas no Brasil - Doutorado