Immunolocalization of markers for bone formation during guided bone regeneration in osteopenic rats

Autor(es)

Tera, Tabata de Mello ^[1] ; Nascimento, Rodrigo Dias ^[2] ; do Prado, Renata Falchete ^[1] ; Santamaria, Mauro Pedrine ^[2] ; Neves Jardini, Maria Aparecida ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Ciencia & Tecnol ICT, Dept Biosci & Oral Diag, Sao Jose Dod Campos, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Ciencia & Tecnol ICT, Dept Diag & Surg, Sao Jose Dod Campos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Oral Science; v. 22, n. 6, p. 541-553, NOV-DEC 2014.
• Citações Web of Science: 6

Resumo

Objective The aim of this paper was to evaluate the repair of onlay autogenous bone grafts covered or not covered by an expanded polytetrafluoroethylene (e-PTFE) membrane using immunohistochemistry in rats with induced estrogen deficiency. Material and Methods Eighty female rats were randomly divided into two groups: ovariectomized (OVX) and with a simulation of the surgical procedure (SHAM). Each of these groups was again divided into groups with either placement of an autogenous bone graft alone (BG) or an autogenous bone graft associated with an e-PTFE membrane (BGM). Animals were euthanized on days 0, 7, 21, 45, and 60. The specimens were subjected to immunohistochemistry for bone sialoprotein (BSP), osteonectin (ONC), and osteocalcin (OCC). Results All groups (OVX+BG, OVX+BMG, SHAM+BG, and SHAM+BMG) showed greater bone formation, observed between 7 and 21 days, when BSP and ONC staining were more intense. At the 45-day, the bone graft showed direct bonding to the recipient bed in all specimens. The ONC and OCC showed more expressed in granulation tissue, in the membrane groups, independently of estrogen deficiency. Conclusions The expression of bone forming markers was not negatively influenced by estrogen deficiency. However, the markers could be influenced by the presence of the e-PTFE membrane. (AU)

• Processo FAPESP: 09/50214-1 - Imunolocalização dos marcadores de formação e reabsorção óssea em regeneração óssea guiada em ratas com osteopenia induzida
• Beneficiário: Maria Aparecida Neves Jardini
• Modalidade de apoio: Auxílio à Pesquisa - Regular