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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

High genital prevalence of cutaneous human papillomavirus DNA on male genital skin: the HPV Infection in Men Study

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Autor(es):
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Sichero, Laura [1] ; Campbell, Christine M. Pierce [2] ; Fulp, William [2] ; Ferreira, Silvaneide [1] ; Sobrinho, Joao S. [1] ; Baggio, Maria Luiza [1] ; Galan, Lenice [3] ; Silva, Roberto C. [4] ; Lazcano-Ponce, Eduardo [5] ; Giuliano, Anna R. [2] ; Villa, Luisa L. [1, 6, 7] ; Grp, HIM Study
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] ICESP, Ctr Translat Oncol, Mol Biol Lab, BR-01246000 Sao Paulo - Brazil
[2] Univ S Florida, Coll Med, H Lee Moffitt Canc Ctr & Res Inst, Ctr Infect Res Canc, Tampa, FL 33612 - USA
[3] Ludwig Inst Canc Res, Sao Paulo - Brazil
[4] Ctr Referencia & Treinamento DST Aids, BR-04121000 Sao Paulo - Brazil
[5] Inst Nacl Salud Publ, Cuernavaca, Morelos - Mexico
[6] Univ Sao Paulo, Sch Med, Dept Radiol & Oncol, Santa Casa De Sao Paulo - Brazil
[7] HPV Inst, Santa Casa De Sao Paulo - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: BMC INFECTIOUS DISEASES; v. 14, DEC 9 2014.
Citações Web of Science: 15
Resumo

Background: The genital skin of males hosts a diversity of HPV genotypes and uncharacterized HPV genotypes. Previously we demonstrated that a specific viral genotype was not identified in 14% of all genital specimens (i.e., HPV unclassified specimens) using the Roche Linear Array method. Our goal was to identify and assess the prevalence of individual HPV types among genital HPV unclassified specimens collected in the HIM Study population, at enrollment, and examine associations with socio-demographic and behavioral characteristics. Methods: Genital skin specimens of men that were considered unclassified (HPV PCR positive, no genotype specified) at enrollment were typed by sequencing amplified PGMY09/11 products or cloning of PGMY/GP+ nested amplicons followed by sequencing. PGMY/GP+ negative specimens were further analyzed using FAP primers. HPV type classification was conducted through comparisons with sequences in the GenBank database. Results: Readable nucleotide sequences were generated for the majority of previously unclassified specimens (66%), including both characterized (77%) and yet uncharacterized (23%) HPV types. Of the characterized HPV types, most (73%) were Beta {[}beta]-HPVs, primarily from beta-1 and beta-2 species, followed by Alpha {[}alpha]-HPVs (20%). Smokers (current and former) were significantly more likely to have an alpha-HPV infection, compared with any other genus; no other factors were associated with specific HPV genera or specific beta-HPV species. Conclusions: Male genital skin harbor a large number of beta-HPV types. Knowledge concerning the prevalence of the diverse HPV types in the men genital is important to better understand the transmission of these viruses. (AU)

Processo FAPESP: 08/57889-1 - Instituto de Ciência e Tecnologia para o Estudo das Doenças Associadas ao Papilomavírus
Beneficiário:Luisa Lina Villa
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 10/15282-3 - Diversidade de tipos de HPV na região genital masculina
Beneficiário:Laura Cristina Sichero Vettorazzo
Linha de fomento: Auxílio à Pesquisa - Regular