Dentifrice pH but not consistency may affect fluoride uptake in plaque

Autor(es)

Cardoso, C. A. B. ^[1] ; Levy, F. M. ^[1] ; Peres-Buzalaf, C. ^[2] ; Buzalaf, M. A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[2] Univ Sagrado Coracao, Ctr Ciencias Saude, BR-17011160 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 43, n. 2, p. 219-224, FEB 2015.
• Citações Web of Science: 2

Resumo

Objective: Test the ability of acidic fluoridated solutions to enhance fluoride (F) bound on bacteria (1) and the effect of dentifrice consistency on plaque fluid F uptake (2). Methods: (1) Streptococcus mutans isolates were grown in BHI medium (37 degrees C/18 h). Bacteria were washed either with EDTA or CaCl2 both at 1 mM to remove or add calcium, respectively. Pellets were incubated with 12 mM NaF at pH 4.5 or 7 for 1 min and F was quantified in the lysates and supernatants with the electrode, after HMDS-facilitated diffusion. (2) A randomized, double-blind, crossover clinical trial was performed in three phases with nineteen adults (20-35 years) that used one of the dentifrices: commercial toothpaste (1100 ppm F, pH7.0 and conventional viscosity (Sorriso Fresh (R))); experimental liquid dentifrice (ELD) (1100 ppm F, pH7.0 and low viscosity {[}1.1% carboxymethylcellulose (CMC)]) and ELD (1100 ppm F and high viscosity pH7.0 (2.2% CMC)). F concentration in plaque fluid was analyzed using an inverted F electrode. Results: (1) Significantly higher F amounts were detected in the lysates of bacteria incubated with NaF solution at pH4.5 compared to the supernatant, which was not seen at pH7.0, being this effect calcium-dependent. (2) Significantly higher F concentrations in plaque fluid were found 1 h after toothbrushing compared to 12 h, but no significant differences were seen among the toothpastes. Conclusions: F at low pH binds more efficiently to S. mutans than at neutral pH and dentifrice viscosity does not interfere in plaque fluid fluoride incorporation. Clinical significance: pH of the dentifrice but not consistency may be important to F uptake in plaque. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/09533-1 - Vernizes contendo xilitol e/ou fluoreto para controle da cárie dentária: estudos in vitro, in situ e clínico randomizado.
• Beneficiário: Cristiane de Almeida Baldini Cardoso
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado