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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Dentifrice pH but not consistency may affect fluoride uptake in plaque

Full text
Author(s):
Cardoso, C. A. B. [1] ; Levy, F. M. [1] ; Peres-Buzalaf, C. [2] ; Buzalaf, M. A. R. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Bauru Dent Sch, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil
[2] Univ Sagrado Coracao, Ctr Ciencias Saude, BR-17011160 Bauru, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Dentistry; v. 43, n. 2, p. 219-224, FEB 2015.
Web of Science Citations: 2
Abstract

Objective: Test the ability of acidic fluoridated solutions to enhance fluoride (F) bound on bacteria (1) and the effect of dentifrice consistency on plaque fluid F uptake (2). Methods: (1) Streptococcus mutans isolates were grown in BHI medium (37 degrees C/18 h). Bacteria were washed either with EDTA or CaCl2 both at 1 mM to remove or add calcium, respectively. Pellets were incubated with 12 mM NaF at pH 4.5 or 7 for 1 min and F was quantified in the lysates and supernatants with the electrode, after HMDS-facilitated diffusion. (2) A randomized, double-blind, crossover clinical trial was performed in three phases with nineteen adults (20-35 years) that used one of the dentifrices: commercial toothpaste (1100 ppm F, pH7.0 and conventional viscosity (Sorriso Fresh (R))); experimental liquid dentifrice (ELD) (1100 ppm F, pH7.0 and low viscosity {[}1.1% carboxymethylcellulose (CMC)]) and ELD (1100 ppm F and high viscosity pH7.0 (2.2% CMC)). F concentration in plaque fluid was analyzed using an inverted F electrode. Results: (1) Significantly higher F amounts were detected in the lysates of bacteria incubated with NaF solution at pH4.5 compared to the supernatant, which was not seen at pH7.0, being this effect calcium-dependent. (2) Significantly higher F concentrations in plaque fluid were found 1 h after toothbrushing compared to 12 h, but no significant differences were seen among the toothpastes. Conclusions: F at low pH binds more efficiently to S. mutans than at neutral pH and dentifrice viscosity does not interfere in plaque fluid fluoride incorporation. Clinical significance: pH of the dentifrice but not consistency may be important to F uptake in plaque. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/09533-1 - XYLITOL- AND/OR FLUORIDE-CONTAINING VARNISHES FOR CARIES CONTROL: IN VITRO, IN SITU AND CLINICAL RANZOMIZED STUDIES.
Grantee:Cristiane de Almeida Baldini Cardoso
Support Opportunities: Scholarships in Brazil - Post-Doctoral