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Evaluation of virulence factors and cell viability of cariogenic bacteria in the presence of Terpinen-4-ol: in vitro study

Grant number: 14/23241-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2015
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Denise Madalena Palomari Spolidorio
Grantee:Ester Alves Ferreira Bordini Galvani
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The use of auxiliary substances in the hygiene, prevention and treatment of oral diseases has been widely spread. The prevention and control of dental plaque should be achieved by mechanical removal and to assist in controlling and reducing the biofilm are recommended mouthwash containing fluoride and antimicrobial agents that may be chemical or natural products. These antimicrobial agents should be effective against microorganisms, act quickly, have no side effects and use without causing discomfort. Alternative therapies with natural extracts open new perspectives for prevention and control of dental disease in search of favorable therapeutic effects. Thus, the Terpinen-4-ol (component of Melaleuca alternifolia oil), membrane-active biocide broad spectrum of action, has been employed as antimicrobial agent and its indications include use in toothpastes without causing bacterial resistance or cytotoxic effects when in contact with the cells of the oral mucosa. This component also has the ability to act in inducing loss of membrane integrity and interfering with the physiology of the microorganism cell. Therefore the objectives of this study are: i) to evaluate the antimicrobial effect of Terpinen-4-ol on cariogenic biofilm on enamel and dentine to Streptococcus mutans and Lactobacillus acidophilus by Confocal Laser Scanning Microscopy (CLSM); ii) to evaluate the virulence factors expressed by these cariogenic bacteria in the presence of Terpinen-4-ol by real-time PCR. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA BORDINI, ESTER ALVES; TONON, CAROLINE CORADI; FRANCISCONI, RENATA SERIGNOLI; CINTRA MAGALHAES, FERNANDO AUGUSTO; MAQUERA HUACHO, PATRICIA MILAGROS; BEDRAN, TELMA LOMBARDO; PRATAVIEIRA, SEBASTIAO; SPOLIDORIO, LUIS CARLOS; SPOLIDORIO, DENISE PALOMARI. Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression. BIOFOULING, v. 34, n. 7, p. 815-825, . (09/54035-4, 15/08742-1, 14/23241-6)
FRANCISCONI, RENATA SERIGNOLI; MAQUERA-HUACHO, PATRICIA MILAGROS; TONON, CAROLINE CORADI; FIORAMONTI CALIXTO, GIOVANA MARIA; ORLANDI SARDI, JANAINA DE CASSIA; CHORILLI, MARLUS; PALOMARI SPOLIDORIO, DENISE MADALENA. Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis. SCIENTIFIC REPORTS, v. 10, n. 1, . (15/08742-1, 14/23241-6, 09/54035-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GALVANI, Ester Alves Ferreira Bordini. Evaluation of virulence factors, antimicrobial activity and cariogenic bacteria cell viability in the presence of Terpinen-4-ol: in vitro study. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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