| Full text | |
| Author(s): |
Ferreira Bordini, Ester Alves
[1]
;
Tonon, Caroline Coradi
[1]
;
Francisconi, Renata Serignoli
[1]
;
Cintra Magalhaes, Fernando Augusto
[1]
;
Maquera Huacho, Patricia Milagros
[1]
;
Bedran, Telma Lombardo
[2]
;
Pratavieira, Sebastiao
[3]
;
Spolidorio, Luis Carlos
[1]
;
Spolidorio, Denise Palomari
[1]
Total Authors: 9
|
| Affiliation: | [1] State Univ Sao Paulo UNESP, Dept Physiol & Pathol, Araraquara, SP - Brazil
[2] Univ Nove de Julho, Dept Periodontol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | BIOFOULING; v. 34, n. 7, p. 815-825, 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. (AU) | |
| FAPESP's process: | 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials |
| Grantee: | Igor Polikarpov |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 15/08742-1 - Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors |
| Grantee: | Denise Madalena Palomari Spolidorio |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/23241-6 - Evaluation of virulence factors and cell viability of cariogenic bacteria in the presence of Terpinen-4-ol: in vitro study |
| Grantee: | Ester Alves Ferreira Bordini Galvani |
| Support Opportunities: | Scholarships in Brazil - Master |