Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression

Author(s)

Ferreira Bordini, Ester Alves ^[1] ; Tonon, Caroline Coradi ^[1] ; Francisconi, Renata Serignoli ^[1] ; Cintra Magalhaes, Fernando Augusto ^[1] ; Maquera Huacho, Patricia Milagros ^[1] ; Bedran, Telma Lombardo ^[2] ; Pratavieira, Sebastiao ^[3] ; Spolidorio, Luis Carlos ^[1] ; Spolidorio, Denise Palomari ^[1]

Affiliation

^[1] State Univ Sao Paulo UNESP, Dept Physiol & Pathol, Araraquara, SP - Brazil

^[2] Univ Nove de Julho, Dept Periodontol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: BIOFOULING; v. 34, n. 7, p. 815-825, 2018.
• Web of Science Citations: 1

Abstract

This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. (AU)

• FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
• Grantee: Igor Polikarpov
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 15/08742-1 - Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors
• Grantee: Denise Madalena Palomari Spolidorio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/23241-6 - Evaluation of virulence factors and cell viability of cariogenic bacteria in the presence of Terpinen-4-ol: in vitro study
• Grantee: Ester Alves Ferreira Bordini Galvani
• Support Opportunities: Scholarships in Brazil - Master