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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression

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Ferreira Bordini, Ester Alves [1] ; Tonon, Caroline Coradi [1] ; Francisconi, Renata Serignoli [1] ; Cintra Magalhaes, Fernando Augusto [1] ; Maquera Huacho, Patricia Milagros [1] ; Bedran, Telma Lombardo [2] ; Pratavieira, Sebastiao [3] ; Spolidorio, Luis Carlos [1] ; Spolidorio, Denise Palomari [1]
Total Authors: 9
[1] State Univ Sao Paulo UNESP, Dept Physiol & Pathol, Araraquara, SP - Brazil
[2] Univ Nove de Julho, Dept Periodontol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Phys Inst Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOFOULING; v. 34, n. 7, p. 815-825, 2018.
Web of Science Citations: 0

This study evaluated the antibacterial activity of terpinen-4-ol against Streptococcus mutans and Lactobacillus acidophilus and its influence on gbpA (S. mutans) and slpA (L. acidophilus) gene expression. As measured by XTT assay, the concentrations of terpinen-4-ol that effectively inhibited the biofilm were 0.24% and 0.95% for S. mutans and L. acidophilus, respectively. Confocal microscopy revealed the presence of a biofilm attached to the enamel and dentin block surfaces with significant terpinen-4-ol effects against these microorganisms. The expression of the gbpA and slpA genes involved in adherence and biofilm formation was investigated using RT-PCR. Expression of these genes decreased after 15min with 0.24% and 0.95% terpinen-4-ol in S. mutans and L. acidophilus, respectively. These findings demonstrate the antimicrobial activity of terpinen-4-ol and its ability to modulate the expression of gbpA and slpA genes, emphasizing the therapeutic capacity of terpinen-4-ol as an alternative to inhibit adherence in biofilm. (AU)

FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support type: Multi-user Equipment Program
FAPESP's process: 14/23241-6 - Evaluation of virulence factors and cell viability of cariogenic bacteria in the presence of Terpinen-4-ol: in vitro study
Grantee:Ester Alves Ferreira Bordini
Support type: Scholarships in Brazil - Master
FAPESP's process: 15/08742-1 - Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors
Grantee:Denise Madalena Palomari Spolidorio
Support type: Regular Research Grants