Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors

• Grant number: 15/08742-1
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2015
• End date: December 31, 2017
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Denise Madalena Palomari Spolidorio
• Grantee: Denise Madalena Palomari Spolidorio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Janaina de Cássia Orlandi Sardi ; Marlus Chorilli

Abstract

Fungal and bacterial infections mouth has been increasingly reported in the literature. Alternative therapies for microbial control are being developed particularly for resistant microorganisms. Plant extracts and phytochemicals compounds open new perspectives for prevention and efficient control of infectious diseases in the search for favorable therapeutic effects. Terpinen-4-ol is a monoterpene that works in induction of membrane loss, affecting the integrity and the physiology of the cell and organism presents broad spectrum of antimicrobial activity and anti-inflammatory. The objectives of this study are: i) to evaluate the synergistic activity of terpinen-4-ol and nystatin, the cytotoxic potential, antifungal property of terpinen-4-ol in nanoparticles form applied in normal oral immortalized cells (NOK), and cell adhesion of Candida albicans after treatment with terpinen-4-ol; ii) to analyze the antibacterial action and the expression of virulence factors of Terpinen-4-ol against cariogenic bacteria; iii) to analyze the decontamination of titanium surfaces with Terpinen-4-ol on the properties of wettability, surface free energy, chemical structure and roughness. Biofilms are prepared using static model microtitre plate and evaluated for cell viability (XTT assay), counting of colony forming units (CFU / mL) and confocal laser microscopy. The antimicrobial activity of Terpinen-4-ol will be determined by microdilution broth method for planktonic cells, biofilms are evaluated by MTT assay for cytotoxicity and NOK cell lines will be assessed by assay of 0.1% sulforodamide B (SRB). In addition, scanning electron microscopy will be used for observation of biofilms and laser confocal microscopy architecture. The synergism of Terpinen-4-ol will be evaluated by the checkerboard methodology. The decontamination protocols of titanium surfaces will be evaluated by atomic force microscopy and photoelectron spectroscopy X-ray. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PATRICIA MILAGROS MAQUERA-HUACHO; GABRIEL GARCIA DE CARVALHO; MIGUEL JAFELICCI JÚNIOR; ELCIO MARCANTONIO JUNIOR; DENISE MADALENA PALOMARI SPOLIDORIO. Physical-chemical influences and cell behavior of natural compounds on titanium dental surfaces. Brazilian Dental Journal, v. 34, n. 5, p. 53-62, 2023-12-22. (15/08742-1)

FRANCISCONI, RENATA SERIGNOLI; MAQUERA-HUACHO, PATRICIA MILAGROS; TONON, CAROLINE CORADI; FIORAMONTI CALIXTO, GIOVANA MARIA; ORLANDI SARDI, JANAINA DE CASSIA; CHORILLI, MARLUS; PALOMARI SPOLIDORIO, DENISE MADALENA. Terpinen-4-ol and nystatin co-loaded precursor of liquid crystalline system for topical treatment of oral candidiasis. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 31 2020. (15/08742-1, 14/23241-6, 09/54035-4)

MAQUERA-HUACHO, PATRICIA MILAGROS; TONON, CAROLINE CORADI; CORREIA, MARILIA FERREIRA; FRANCISCONI, RENATA SERIGNOLI; FERREIRA BORDINI, ESTER ALVES; MARCANTONIO, ELCIO; PALOMARI SPOLIDORIO, DENISE MADALENA. In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces. BIOFOULING, v. 34, n. 6, p. 699-709, 2018. (09/54035-4, 15/08742-1)

FERREIRA BORDINI, ESTER ALVES; TONON, CAROLINE CORADI; FRANCISCONI, RENATA SERIGNOLI; CINTRA MAGALHAES, FERNANDO AUGUSTO; MAQUERA HUACHO, PATRICIA MILAGROS; BEDRAN, TELMA LOMBARDO; PRATAVIEIRA, SEBASTIAO; SPOLIDORIO, LUIS CARLOS; SPOLIDORIO, DENISE PALOMARI. Antimicrobial effects of terpinen-4-ol against oral pathogens and its capacity for the modulation of gene expression. BIOFOULING, v. 34, n. 7, p. 815-825, 2018. (09/54035-4, 15/08742-1, 14/23241-6)