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Analyses of cytotoxicity of Terpinen-4-ol in the precursor liquid crystal system

Grant number: 15/26702-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2016
Effective date (End): October 31, 2017
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Denise Madalena Palomari Spolidorio
Grantee:Juliane de Camargo Zatiti
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Candida spp. is a fungal pathogen that has the capacity to form oral biofilms. Systemic and extrinsic factors such as poor oral hygiene, the use of ill-fitting dentures, and little salivation are predisposing to the development of candidiasis. The development of new treatment options has been studied since there is a need to limit the resistance created by the fungi to existing antifungals. Thus, herbal medicine presents potentially valuable and among the herbal medicines, there is the Terpinen-4-ol with antimicrobial and anti-inflammatory action. The delivery of the drug system directly influences the efficiency of the treatment and must aim at increasing the local bioavailability for a certain period of time. The liquid crystal precursor system provides slow and durable release of the drug on the microorganisms, being thus an effective alternative in the treatment of infections. The objective of this study is to evaluate the cytotoxic and determine the non-cytotoxic concentrations of the liquid crystal precursor system in association with the Terpinen-4-ol. Will be used cell culture immortalized line of human keratinocytes (NOK). The cells will be grown in a culture medium Dulbecco's Eagle's Modified Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin, streptomycin, and glutamine and kept in an incubator at 37 C in 5% CO2. The cytotoxicity of Terpinen-4-ol will be evaluated using the colorimetric assay MTT (3- (4,5-dimethylthiazol-2-yl) -2,5-Diphenyltetrazolium Bromide) for evaluation of cell viability and proliferation by cytochemistry of enzyme succinic dehydrogenase activity (SDH). The results will be evaluated using the SPSS software version 17.0 (Chicago, IL, USA). The data for the cellular metabolism will be evaluated at a significance level of 5% (p <0.05).(AU)

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