Molecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum

Autor(es)

Passarini, Michel R. Z. ^[1] ; Miqueletto, Paula B. ^[1] ; de Oliveira, Valeria M. ^[1] ; Sette, Lara D. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CPQBA, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Div Recursos Microbianos, UNICAMP, Paulinia, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF BASIC MICROBIOLOGY; v. 55, n. 2, p. 207-220, FEB 2015.
• Citações Web of Science: 8

Resumo

The present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples. (AU)

• Processo FAPESP: 08/06720-7 - Caracterização da diversidade de fungos filamentosos associados a esponjas marinhas e avaliação da produção de lacase
• Beneficiário: Michel Rodrigo Zambrano Passarini
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/50190-2 - Investigação do potencial biotecnológico e metabólico de organismos marinhos para processos de biorremediação e produção de substâncias com atividades anti-virais, anti-leishmania e anti-inflamatória (Biodiversidade Marinha)
• Beneficiário: Roberto Gomes de Souza Berlinck
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático