Molecular diversity of fungal and bacterial communities in the marine sponge Dragmacidon reticulatum

Author(s)

Passarini, Michel R. Z. ^[1] ; Miqueletto, Paula B. ^[1] ; de Oliveira, Valeria M. ^[1] ; Sette, Lara D. ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, CPQBA, Ctr Pluridisciplinar Pesquisas Quim Biol & Agr, Div Recursos Microbianos, UNICAMP, Paulinia, SP - Brazil

^[2] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Bioquim & Microbiol, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF BASIC MICROBIOLOGY; v. 55, n. 2, p. 207-220, FEB 2015.
• Web of Science Citations: 8

Abstract

The present work aimed to investigate the diversity of bacteria and filamentous fungi of southern Atlantic Ocean marine sponge Dragmacidon reticulatum using cultivation-independent approaches. Fungal ITS rDNA and 18S gene analyses (DGGE and direct sequencing approaches) showed the presence of representatives of three order (Polyporales, Malasseziales, and Agaricales) from the phylum Basidiomycota and seven orders belonging to the phylum Ascomycota (Arthoniales, Capnodiales, Dothideales, Eurotiales, Hypocreales, Pleosporales, and Saccharomycetales). On the other hand, bacterial 16S rDNA gene analyses by direct sequencing approach revealed the presence of representatives of seven bacterial phyla (Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Lentisphaerae, Chloroflexi, and Planctomycetes). Results from statistical analyses (rarefaction curves) suggested that the sampled clones covered the fungal diversity in the sponge samples studied, while for the bacterial community additional sampling would be necessary for saturation. This is the first report related to the molecular analyses of fungal and bacterial communities by cultivation-independent approaches in the marine sponges D. reticulatum. Additionally, the present work broadening the knowledge of microbial diversity associated to marine sponges and reports innovative data on the presence of some fungal genera in marine samples. (AU)

• FAPESP's process: 08/06720-7 - Characterization of filamentous fungi diversity from marine sponges and evaluation of laccase production
• Grantee: Michel Rodrigo Zambrano Passarini
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/50190-2 - Investigation of metabolic and biotechnological potential of marine organisms in bioremediation processes and for the production of substances with anti-viral, anti-inflammatory and anti-Leishmania activities (Marine Biodiversity)
• Grantee: Roberto Gomes de Souza Berlinck
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants