Holobiome of the marine sponges, effects of the climate changes and biotechnological potentials: a holistic and multidisciplinary approach

• Grant number: 16/17189-7
• Support Opportunities: Research Program on Global Climate Change - Young Investigators
• Start date: July 01, 2017
• End date: August 31, 2022
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Cristiane Cassiolato Pires Hardoim
• Grantee: Cristiane Cassiolato Pires Hardoim
• Host Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil
• Associated researchers: Tânia Marcia Costa ; Wagner Vilegas
• Associated scholarship(s): 19/08876-9 - Screening of compounds with anticancer activities produced by bacteria associated with marine sponges from São Paulo coast, BP.MS; 19/07957-5 - Reveal the controlling factors of the Aplysina fulva, A. caissara and A. cauliformis communities on the coast of São Paulo, BP.MS; 19/07646-0 - Holobiome of the marine sponges, effects of the climate changes and biotechnological potentials: a holistic and multidisciplinary approach, BP.TT; + associated scholarships 19/01769-2 - Isolation, characterization and analysis of the capacity to produce antimicrobials substances from bacteria associated with Aplysina caissara, BP.IC; 18/12937-0 - Holobiome of the marine sponges, effects of the climate changes and biotechnological potentials: a holistic and multidisciplinary approach, BP.TT; 17/10157-5 - Holobiome of the marine sponges, effects of the climate changes and biotechnological potentials: a holistic and multidisciplinary approach, BP.JP.PFPMCG - associated scholarships

Abstract

Marine sponges perform several services that contribute to the functioning and health of the benthic ecosystems. They also harbor diverse bacterial communities in which complex interactions are established and aid the host to grow and develop. The marine sponges are since 1970´s the main sources of biotechnological products and it is known that several of bioactive compounds obtained from these animals are, actually, synthesized by the microbial communities associated. Conversely, little is still known about the diversity and community structure of the archaeal and eukaryotic associated with marine sponges, although Archaea are also involved in the nitrogen cycle. This observation emphasizes the need to disclose their taxonomic and functional diversities within these animals. Nowadays, like many other marine creatures, sponges also face numerous threats mainly due to the effects of the climate changes. The species Aplysina fulva, A. caissara (endemic) and A. cauliformis that occur in the coast of São Paulo state and are known for their high capacity to form associations with diverse microorganisms will be investigated in this proposal by using ecological, microbiological and biotechnological approaches. It will be determined if the top-down factor controls the growth of the sponges in the coast of São Paulo. Culture-dependent and -independent methods will be applied to characterize the prokaryotic and eukaryotic communities associated with these sponges. Taxonomic diversity and community structure will be assessed by high-throughput sequencing of phylogenetic markers 16S and 18S rRNA genes. These sponge species will be collected along with surrounding seawater and sediment to verify the specificity of the microbial communities associated with the investigated sponges. The laboratory experiments using temperature expected in future scenarios of climate change will rely on the know-how from Prof. Dr. Tânia Costa for its execution. These effects in the prokaryotic community and in the host will be investigated by metagenomic and metatranscriptome analyses. Besides, the temporal stability of associated prokaryotic community will be surveyed in situ along four years for the three Aplysina species. The biotechnological potential of the bacteria isolated from three Aplysina species will be evaluated for the production of antimicrobial and anticancer compounds. Researchers from the laboratory of Prof. Dr. Wagner Vilegas will perform the bioprospecting and the genomic sequencing of selected bacteria with antimicrobial or anticancer activities. The results obtained in this project will be innovative for sponges of South Atlantic and will enable to foster our understanding on the ecological roles of both host sponges and associated microbial symbionts in the benthic ecosystem. Besides, this project will assess the great biotechnological potential of the bacterial isolated from marine sponges before the shift in seawater temperature cause the loss of the biodiversity of these animals. Thus, the question of this proposal is to assess the biodiversity and functionality of the sponge holobiome in the cost of São Paulo. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NASCIMENTO-SILVA, GABRIEL; COSTA, CAROLINA MENDES; LOBO-HAJDU, GISELE; CUSTODIO, MARCIO REIS; HARDOIM, CRISTIANE CASSIOLATO PIRES. Diversity and structure of bacterial and archaeal communities associated with the vulnerable sponge Halichondria cebimarensis. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. N/A, p. 16-pg., 2023-01-16. (16/17189-7, 19/04707-8, 19/07646-0, 17/10157-5)

DEL CASTILO, INGRID; NEUMANN, ARTHUR S.; LEMOS, FELIPE S.; DE BASTIANI, MARCO A.; OLIVEIRA, FELIPE L.; ZIMMER, EDUARDO R.; REGO, AMANDA M.; HARDOIM, CRISTIANE C. P.; ANTUNES, LUIS CAETANO M.; LARA, FLAVIO A.; et al. Lifelong Exposure to a Low-Dose of the Glyphosate-Based Herbicide RoundUp (R) Causes Intestinal Damage, Gut Dysbiosis, and Behavioral Changes in Mice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 10, p. 17-pg., 2022-05-01. (17/10157-5, 16/17189-7)

HARDOIM, CRISTIANE C. P.; LOBO-HAJDU, GISELE; CUSTODIO, MARCIO R.; HARDOIM, PABLO R.. Prokaryotic, Fungal, and Unicellular Eukaryotic Core Communities Across Three Sympatric Marine Sponges From the Southwestern Atlantic Coast Are Dominated Largely by Deterministic Assemblage Processes. FRONTIERS IN MICROBIOLOGY, v. 12, JUN 8 2021. (17/10157-5, 16/17189-7)

NASCIMENTO-SILVA, GABRIEL; HARDOIM, CRISTIANE CASSIOLATO PIRES; CUSTODIO, MARCIO REIS. The Porifera microeukaryome: Addressing the neglected associations between sponges and protists. MICROBIOLOGICAL RESEARCH, v. 265, p. 17-pg., 2022-12-01. (19/04707-8, 16/17189-7, 17/10157-5)

HARDOIM, CRISTIANE C. P.; HARDOIM, PABLO R.; LOBO-HAJDU, GISELE; CUSTODIO, MARCIO R.; THOMAS, TORSTEN. The microbiome of the sponge Aplysina caissara in two sites with different levels of anthropogenic impact. FEMS Microbiology Letters, v. 370, p. 8-pg., 2023-01-17. (16/17189-7, 18/12937-0, 17/10157-5)

HARDOIM, C. C. P.; RAMAGLIA, A. C. M.; LOBO-HAJDU, G.; CUSTODIO, M. R.. Community composition and functional prediction of prokaryotes associated with sympatric sponge species of southwestern Atlantic coast. SCIENTIFIC REPORTS, v. 11, n. 1, MAY 5 2021. (16/17189-7, 17/10157-5, 18/12937-0, 17/11380-0)