MxA interacts with and is modified by the SUMOylation machinery

Autor(es)

Brantis-de-Carvalho, Carlos Eduardo ^{[1, 2]} ; Maarifi, Ghizlane ^[1] ; Goncalves Boldrin, Paulo Eduardo ^[2] ; Zanelli, Cleslei Fernando ^[2] ; Nisole, Sebastien ^[1] ; Chelbi-Alix, Mounira K. ^[1] ; Valentini, Sandro Roberto ^[2]

Afiliação do(s) autor(es)

^[1] Univ Paris 05, INSERM, UMR S 1124, F-75006 Paris - France

^[2] Univ Estadual Paulista UNESP, Sch Pharmaceut Sci, Dept Biol Sci, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Cell Research; v. 330, n. 1, p. 151-163, JAN 1 2015.
• Citações Web of Science: 9

Resumo

Mx proteins are evolutionarily conserved dynamin-like large GTPases involved in viral resistance triggered by types I and III interferons. The human MxA is a cytoplasmic protein that confers resistance to a large number of viruses. The MxA protein is also known to self-assembly into high molecular weight homo-oligomers. Using a yeast two-hybrid screen, we identified 27 MxA binding partners, some of which are related to the SUMOylation machinery. The interaction of MxA with Small-Ubiquitin MOdifier 1 (SUMO1) and Ubiquitin conjugating enzyme 9 (Ubc9) was confirmed by co-immunoprecipitation and co-localization by confocal microscopy. We identified one SUMO conjugation site at lysine 48 and two putative SUMO interacting motifs (SIMa and SIMb). We showed that MxA interacts with the EIL loop of SUMO1 in a SIM-independent manner via its CID-GED domain. The yeast two-hybrid mapping also revealed that Ubc9 binds to the MxA GTPase domain. Mutation in the putative SIMa and SIMb, which are located in the GTPase binding domain, reduced MxA antiviral activity. In addition, we showed that MxA can be conjugated to SUMO2 or SUMO3 at lysine 48 and that the SUMOylation-deficient mutant of MxA (MxA(K48R)) retained its capacity to oligomerize and to inhibit Vesicular Stomatitis Virus (VSV) and Influenza A Virus replication, suggesting that MxA SUMOylation is not essential for its antiviral activity. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 03/09497-3 - Identificação e caracterização funcional de marcadores moleculares envolvidos no processo de formação de tumores de cabeça e pescoço por meio da análise do padrão diferencial de metilação
• Beneficiário: Sandro Roberto Valentini
• Linha de fomento: Auxílio à Pesquisa - Temático
• Processo FAPESP: 10/50044-6 - Controle da expressão gênica em nível traducional: estudo do papel de elF5A na elongação da tradução
• Beneficiário: Sandro Roberto Valentini
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