Combination of hyperthermia and photodynamic therapy on mesenchymal stem cell line treated with chloroaluminum phthalocyanine magnetic-nanoemulsion

Autor(es)

de Paula, Leonardo B. ^{[1, 2]} ; Primo, Fernando L. ^{[2, 3]} ; Pinto, Marcelo R. ^[4] ; Morais, Paulo C. ^{[5, 6]} ; Tedesco, Antonio C. ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidual, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Nanophotons Co, SUPERA Innovat & Technol Pk, BR-14056680 Ribeirao Preto - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Enzymol Lab, BR-14040901 Ribeirao Preto, SP - Brazil

^[5] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF - Brazil

^[6] Huazhong Univ Sci & Technol, Sch Automat, Wuhan 430074 - Peoples R China

• Tipo de documento: Artigo Científico
• Fonte: Journal of Magnetism and Magnetic Materials; v. 380, p. 372-376, APR 15 2015.
• Citações Web of Science: 21

Resumo

The present study reports On the preparation and the cell viability assay of two nanoemulsions loaded with magnetic nanoparticle and chloroaluminum phthalocyanine. The preparations contain equal amount of chloroaluminum phthalocyanine (0.05 mg/mL) but different contents of magnetic nanoparticle (0.15 x 10(13) or 1.50 x 10(13) particle/mL). The human bone marrow mesenchymal stem cell line was used as the model to assess the cell viability and this type of cell can be used as a model to mimic cancer stem cells. The cell viability assays were performed in isolated as well as under combined magnetic hyperthermia and photodynamic therapy treatments. We found from the cell viability assay that under the hyperthermia treatment (1 MHz and 40 Oe magnetic field amplitude) the cell viability reduction was about 10%, regardless the magnetic nanoparticle content within the magnetic nanoparticle/ chloroaluminum phthalocyanine formulation. However, cell viability reduction of about 50% and 60% were found while applying the photoclynamic therapy treatment using the magnetic nanoparticle/chloroaluminum phthalocyanine formulation containing 0.15 x 10(13) or 1.50 x 10(13) magnetic particle/mL, respectively. Finally, an average reduction in cell viability of about 66% was found while combining the hyperthermia and photoclynamic therapy treatments. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 14/14231-7 - Desenvolvimento de dispositivos poliméricos nanobiocarreadores para liberação controlada de fármacos fotossensibilizadores ftalocianínicos aplicados à regeneração de tecidos biológicos e tratamento de lesões
• Beneficiário: Fernando Lucas Primo
• Modalidade de apoio: Bolsas no Brasil - Pesquisa Inovativa em Pequenas Empresas - PIPE
• Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático