Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Combination of hyperthermia and photodynamic therapy on mesenchymal stem cell line treated with chloroaluminum phthalocyanine magnetic-nanoemulsion

Full text
Author(s):
de Paula, Leonardo B. [1, 2] ; Primo, Fernando L. [2, 3] ; Pinto, Marcelo R. [4] ; Morais, Paulo C. [5, 6] ; Tedesco, Antonio C. [2, 3]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, BR-14040901 Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ctr Nanotecnol & Engn Tecidual, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Nanophotons Co, SUPERA Innovat & Technol Pk, BR-14056680 Ribeirao Preto - Brazil
[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Enzymol Lab, BR-14040901 Ribeirao Preto, SP - Brazil
[5] Univ Brasilia, Inst Fis, BR-70910900 Brasilia, DF - Brazil
[6] Huazhong Univ Sci & Technol, Sch Automat, Wuhan 430074 - Peoples R China
Total Affiliations: 6
Document type: Journal article
Source: Journal of Magnetism and Magnetic Materials; v. 380, p. 372-376, APR 15 2015.
Web of Science Citations: 21
Abstract

The present study reports On the preparation and the cell viability assay of two nanoemulsions loaded with magnetic nanoparticle and chloroaluminum phthalocyanine. The preparations contain equal amount of chloroaluminum phthalocyanine (0.05 mg/mL) but different contents of magnetic nanoparticle (0.15 x 10(13) or 1.50 x 10(13) particle/mL). The human bone marrow mesenchymal stem cell line was used as the model to assess the cell viability and this type of cell can be used as a model to mimic cancer stem cells. The cell viability assays were performed in isolated as well as under combined magnetic hyperthermia and photodynamic therapy treatments. We found from the cell viability assay that under the hyperthermia treatment (1 MHz and 40 Oe magnetic field amplitude) the cell viability reduction was about 10%, regardless the magnetic nanoparticle content within the magnetic nanoparticle/ chloroaluminum phthalocyanine formulation. However, cell viability reduction of about 50% and 60% were found while applying the photoclynamic therapy treatment using the magnetic nanoparticle/chloroaluminum phthalocyanine formulation containing 0.15 x 10(13) or 1.50 x 10(13) magnetic particle/mL, respectively. Finally, an average reduction in cell viability of about 66% was found while combining the hyperthermia and photoclynamic therapy treatments. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 14/14231-7 - Development of polymeric nanocarriers for controlled release devices photosensitizer drug phthalocyanines applied to biological tissue regeneration and treatment of lesions
Grantee:Fernando Lucas Primo
Support Opportunities: Scholarships in Brazil - Innovative Research in Small Business - PIPE
FAPESP's process: 08/53719-4 - Use of nanocarriers containing photosensitive dyes and other derived and growing factors applied to the photodynamic process in the health areas
Grantee:Antonio Claudio Tedesco
Support Opportunities: Research Projects - Thematic Grants