Organic and Inorganic Fractions of Diesel Exhaust Particles Produce Changes in Mucin Profile of Mouse Trachea Explants

Autor(es)

Seriani, Robson ^[1] ; Junqueira, Mara S. ^[2] ; Toledo, Alessandra C. ^[3] ; Correa, Aristides T. ^[4] ; Silva, Luiz F. F. ^[1] ; Martins, Milton A. ^[3] ; Saldiva, Paulo H. N. ^[1] ; Mauad, Thais ^[1] ; Macchione, Mariangela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Pathol, Lab Expt Air Pollut, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Cent Biotery Lab, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Fac Clin Med, Expt Therapeut Lab, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Heart Inst InCor, Sch Med, Dept Cardiopulmonol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES; v. 78, n. 4, p. 215-225, FEB 16 2015.
• Citações Web of Science: 5

Resumo

Diesel exhaust particles (DEP) contain organic and inorganic elements that produce damage to the respiratory epithelium. The aim of this study was to determine the mucus profile of tracheal explants exposed to either crude diesel exhaust particles (DEP) or DEP treated with nitric acid (DEP/NA), with hexane (DEP/HEX), or with methanol (DEP/MET) at concentrations of 50 and 100 mu g/ml for 30 and 60 min. Tracheal explants were subjected to morphometric analyses to study acidic (AB+), neutral (PAS+), and mixed (AB+/PAS+) mucus production and vacuolization (V). Incubation with 50 mu g/ml crude DEP resulted in a rise in acid mucus production, an increase in vacuolization at 30 min, and reduction in neutral mucus at 30 and 60 min. Tracheas exposed to DEP/MET at 50 mu g/ml for 30 or 60 min resulted in a significant decrease in neutral mucus production and an elevation in acid mucus production. DEP/HEX increased vacuolization at both 50 and 100 mu g/ml at 30 and 60 min of exposure. Treatment with 50 mu g/ml for 30 or 60 min significantly elevated mixed mucus levels. These results suggest that DEP appear to be more toxic when administered in combination with HEX or MET. DEP/MET modified the mucus profile of the epithelium, while DEP/HEX altered mucus extrusion, and these responses might be due to bioavailability of individual elements in DEP fractions. (AU)

• Processo FAPESP: 11/50334-7 - Avaliação da toxicidade do material particulado urbano em cultura de células do epitélio brônquico humano: estudo da expressão gênica de enzimas antioxidante e sinalização celular
• Beneficiário: Robson Seriani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto