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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Monocyte-derived dendritic cells reflect the immune functional status of a chromophobe renal cell carcinoma patient: Could it be a general phenomenon?

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Autor(es):
Clavijo-Salomon, Maria A. [1] ; Ramos, Rodrigo N. [1] ; Crippa, Alexandre [2] ; Pizzo, Celia R. [1] ; Bergami-Santos, Patricia C. [1] ; Barbuto, Jose Alexandre M. [1, 3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Lab Tumor Immunol, Dept Immunol, Inst Biomed Sci, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Med, Sao Paulo State Canc Inst ICESP, Sect Urooncol, Div Urol, Sao Paulo - Brazil
[3] Univ Sao Paulo, Cell & Mol Therapy Ctr NUCEL NETCEM, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: CANCER IMMUNOLOGY IMMUNOTHERAPY; v. 64, n. 2, p. 161-171, FEB 2015.
Citações Web of Science: 4
Resumo

The chromophobe renal cell carcinoma (ChRCC), though associated with a hereditary cancer syndrome, has a good prognosis after tumor removal. The lack of recurrence could be related to the absence of immune system compromise in patients or to an effective functional recovery of immune functions after tumor removal. Thus, we evaluated monocyte-derived dendritic cells (Mo-DCs) in a 34-year-old male who had a ChRCC, before and after tumor removal. CD14(+) monocytes from the patient's peripheral blood, 1 week before and 3 months after partial nephrectomy, were differentiated in vitro into immature and mature Mo-DCs. These were harvested, analyzed by flow cytometry and used as stimulators of allogeneic T cells. Supernatants from cultures were collected for cytokine analysis. Tumor removal was associated with decreased expression of PD-L1, but also, surprisingly, of CD205, HLA-DR, CD80 and CD86 by Mo-DCs. Also, Mo-DC's ability to stimulate T cell proliferation increased, along with IL-2R alpha expression and IFN-gamma production. Simultaneously, the patients' Mo-DCs ability to induce Foxp3(+) T cells decreased after surgery. One-year postoperative follow-up shows no tumor recurrence. The presence of a ChRCC affected Mo-DCs generated in vitro, which recovered their function after tumor removal. This indicates that the favorable outcome observed after ChRCC resection may be due to the restoration of immunocompetence. Furthermore, since functional alterations described for DCs within tumors may be also found in Mo-DCs, their accurate functional analysis-not restricted to the determination of their surface immunophenotype-may provide an indirect ``window{''} to the tumor microenvironment. (AU)

Processo FAPESP: 12/23478-0 - Efeito das células Natural Killer sobre a diferenciação in vitro de monócitos em células dendríticas em pacientes com câncer: estudo de mecanismos envolvidos
Beneficiário:Maria Alejandra Clavijo Salomón
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 09/54599-5 - Células dendríticas: elementos integrados do sistema imune - enfoque aplicado
Beneficiário:Jose Alexandre Marzagão Barbuto
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/05331-0 - Estudo do efeito das células Natural Killer sobre a diferenciação in vitro de monócitos em células dendríticas em pacientes com câncer
Beneficiário:Maria Alejandra Clavijo Salomón
Linha de fomento: Bolsas no Brasil - Mestrado