Low genetic diversity and intrapopulation spatial genetic structure of the Atlantic Forest tree, Esenbeckia leiocarpa Engl. (Rutaceae)

Autor(es)

Forti, G. ^[1] ; Tambarussi, E. V. ^[2] ; Kageyama, P. Y. ^[2] ; Moreno, M. A. ^[2] ; Ferraz, E. M. ^[2] ; Ibanes, B. ^[2] ; Mori, G. M. ^{[3, 4]} ; Vencovsky, R. ^[2] ; Sebbenn, A. M. ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Univ Metodista Piracicaba Unimep, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil

^[4] Agencia Paulista Tecnol Agronegocios, Polo Reg Ctr Sul, BR-13400970 Piracicaba, SP - Brazil

^[5] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ANNALS OF FOREST RESEARCH; v. 57, n. 2, p. 165-174, 2014.
• Citações Web of Science: 7

Resumo

Studies on population genetics are the key to designing effective in situ management plans for tree species, in particular, those subjected to pressure from anthropogenic processes, such as forest fragmentation and logging. To investigate genetic diversity, inbreeding and intrapopulation spatial genetic structure (SGS) in a fragmented population of the insect-pollinated tropical tree, Esenbeckia leiocarpa, we developed specific microsatellite markers for this species and mapped and sampled 100 individuals in a forest plot. Two issues were addressed in particular: (i) the level of genetic diversity, inbreeding and effective population size, (ii) whether intrapopulation spatial genetic structure exists. Among the 14 loci developed, we only used the three that presented polymorphism to estimate the genetic parameters. Genetic diversity was low, whereby the average number of alleles per locus (A) was 3.3 and observed (H-0) and expected heterozygosities (H were 0.336 and 0.298, respectively. The average fixation index was significantly higher than zero (F = 0.112), suggesting inbreeding. Significant SGS was found up to 7 m and between 31 to 38 m, indicating that trees growing within these distances may be related. Estimates of the effective population size indicated that the 100 sampled trees correspond to 14 individuals that are neither related nor inbred. Our results suggest that the microsatellite markers developed in this study are suitable for studies on genetic diversity and structure, mating systems, gene flow and SGS in this species. (AU)

• Processo FAPESP: 12/20790-3 - Desenvolvimento e caracterização de locos microssatélites de guarantã (Esenbeckia leiocarpa Engl.) visando estudos genéticos e ecológicos
• Beneficiário: Giullia Forti
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 10/50033-4 - Caracterização de populações naturais de Avicennia germinans e de A. shaueriana (Acanthaceae) de manguezais do litoral norte brasileiro e análise de zona de hibridação utilizando marcadores
• Beneficiário: Gustavo Maruyama Mori
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/12354-3 - Fluxo gênico contemporâneo, sistema de reprodução e estrutura espacial de genótipos em população fragmentada de jequitibá-rosa (Cariniana legalis Mart. O. Kuntze) utilizando marcadores microssatélites.
• Beneficiário: Evandro Vagner Tambarussi
• Modalidade de apoio: Bolsas no Brasil - Doutorado