Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever

Autor(es)

Gentile Cury, Gisele Cristiane ^[1] ; Carneiro Pereira, Rafaella Fabiana ^[1] ; de Hollanda, Luciana Maria ^[1] ; Lancellotti, Marcelo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Biochem, Biotechnol Lab, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 45, n. 4, p. 1449-1454, OCT-DEC 2014.
• Citações Web of Science: 0

Resumo

The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae. (AU)

• Processo FAPESP: 08/56777-5 - Estudo molecular e profilático das bactérias naturalmente transformaveis do trato respiratório humano - Haemophilus influenzae, Neisseria meningitidis e Streptococcus pneumoniae
• Beneficiário: Marcelo Lancellotti
• Modalidade de apoio: Auxílio à Pesquisa - Regular