Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex

Autor(es)

Prieto, Marcela B. ^[1] ; Georg, Raphaela C. ^[1] ; Gonzales-Zubiate, Fernando A. ^[1] ; Luz, Juliana S. ^[1] ; Oliveira, Carla C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MOLECULAR BIOLOGY; v. 16, MAR 18 2015.
• Citações Web of Science: 9

Resumo

Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits. (AU)

• Processo FAPESP: 10/51842-3 - SMOLBNET2: estudo de estrutura de proteínas componentes e reguladoras do exossomo de Archaea e de levedura
• Beneficiário: Carla Columbano de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/51200-7 - Identificação e determinação da localização celular das subunidades do exossomo em testículos de Lithobates catesbeianus (rã touro)
• Beneficiário: Juliana Silva da Luz
• Modalidade de apoio: Auxílio à Pesquisa - Regular