Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex

Author(s)

Prieto, Marcela B. ^[1] ; Georg, Raphaela C. ^[1] ; Gonzales-Zubiate, Fernando A. ^[1] ; Luz, Juliana S. ^[1] ; Oliveira, Carla C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Chem, Dept Biochem, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: BMC MOLECULAR BIOLOGY; v. 16, MAR 18 2015.
• Web of Science Citations: 9

Abstract

Background: Box C/D snoRNPs are responsible for rRNA methylation and processing, and are formed by snoRNAs and four conserved proteins, Nop1, Nop56, Nop58 and Snu13. The snoRNP assembly is a stepwise process, involving other protein complexes, among which the R2TP and Hsp90 chaperone. Nop17, also known as Pih1, has been shown to be a constituent of the R2TP (Rvb1, Rvb2, Tah1, Pih1) and to participate in box C/D snoRNP assembly by its interaction with Nop58. The molecular function of Nop17, however, has not yet been described. Results: To shed light on the role played by Nop17 in the maturation of snoRNP, here we analyzed the interactions domains of Nop58 - Nop17 - Tah1 and the importance of ATP to the interaction between Nop17 and the ATPase Rvb1/2. Conclusions: Based on the results shown here, we propose a model for the assembly of box C/D snoRNP, according to which R2TP complex is important for reducing the affinity of Nop58 for snoRNA, and for the binding of the other snoRNP subunits. (AU)

• FAPESP's process: 10/51842-3 - Structural study of protein components of the exosome and some of its regulatory factors from Archaea and yeast
• Grantee: Carla Columbano de Oliveira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/51200-7 - Identification and cellular localization of the exosome subunits in testis of Lithobates catesbeianus (Bullfrog)
• Grantee: Juliana Silva da Luz
• Support Opportunities: Regular Research Grants