Structural study of protein components of the exosome and some of its regulatory factors from Archaea and yeast

• Grant number: 10/51842-3
• Support Opportunities: Research Projects - Thematic Grants
• Duration: January 01, 2011 - December 31, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Carla Columbano de Oliveira
• Grantee: Carla Columbano de Oliveira
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Nilson Ivo Tonin Zanchin
• Associated grant(s): 12/21016-0 - Visit to labs at the Chemistry Institute and at the Biosciences Institute of USP to establish collaborations in systems biology and analysis of protein interactions, to coordinate graduate students exchange between USP and UK, and to participate in SBBq, AV.EXT
• Associated scholarship(s): 15/05776-2 - Functional characterization of Nop53/PICT1: modulation of ribosome biogenesis, the exosome activity, and effect on cell cycle, BP.DD; 14/04628-7 - Exosome cofactors role analysis in RRP6 exoribonuclease expression and stability in Saccharomyces cerevisiae, BP.IC; 12/50196-6 - Biochemical and structural characterization of yeast exosome interactions with regulatory proteins of their activity, BP.PD; 11/04073-7 - Genetics aspects of primary open-angle glaucoma(POAG)in the brazilian population, BP.DR

Abstract

In eukaryotes, many posttranscriptional processing events are necessary for the synthesis of mature RNAs. mRNAs, tRNAs, rRNAs, snRNAs and snoRNAs are processed through several steps that involve specific reactions between RNA and proteins. Although most processing factors are specific for each pathway, some of those involved in the maturation of the different kinds of RNAs are common to all pathways. Among the common factors is the exosome, a complex formed by eleven subunits in Saccharomyces cerevisiae and humans that shows 3' to 5' exonucleolytic RNase activity and that is involved in the maturation of rRNAs, snoRNAs, and snRNAs and in the degradation of all classes of RNA, including the RNAi pathway. The main focus of our laboratory has been the study of the posttranscriptional control of gene expression through the functional and structural characterization of proteins involved in RNA processing and degradation. We have previously determined the structure of the exosome complex in the archaea Pyrococcus based on small angle X-ray scattering (Ramos et al., 2006) and on crystallography data (Navarro et al., 2008). We have also obtained a model of the exosome architecture in eukaryotes based on the yeast subunits interaction data (Luz et al., 2007). In addition, we have studied exosome regulatory factors in yeast (Granato el al., 2008) and in Pyrococcus abyssi (Luz et al., 2010). The main goal or this work is to continue studying the exosome structure and function in yeast and archaea and also determine how proteins interacting with this complex might affect its general structure. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PRIETO, MARCELA B.; GEORG, RAPHAELA C.; GONZALES-ZUBIATE, FERNANDO A.; LUZ, JULIANA S.; OLIVEIRA, CARLA C.. Nop17 is a key R2TP factor for the assembly and maturation of box C/D snoRNP complex. BMC MOLECULAR BIOLOGY, v. 16, MAR 18 2015. (10/51842-3, 12/51200-7)

GONZALES-ZUBIATE, FERNANDO A.; OKUDA, ELLEN K.; DA CUNHA, JULIA P. C.; OLIVEIRA, CARLA COLUMBANO. Identification of karyopherins involved in the nuclear import of RNA exosome subunit Rrp6 in Saccharomyces cerevisiae. Journal of Biological Chemistry, v. 292, n. 29, p. 12267-12284, JUL 21 2017. (12/50196-6, 10/51842-3, 15/06477-9)

LUZ, J. S.; CANEGUIM, B. H.; BAGGIO, A.; SANTONI, M. M.; HELBING, C. C.; VALENTINI, S. R.; SASSO-CERRI, E.; OLIVEIRA, C. C.. Differential expression of RNA exosome subunits in the amphibian Lithobates catesbeianus during reproductive and non-reproductive periods. BMC RESEARCH NOTES, v. 12, p. 7-pg., 2019-01-21. (12/51200-7, 12/23845-3, 14/21383-8, 10/51842-3)

LOURENCO, ROGERIO F.; LEME, ADRIANA F. P.; OLIVEIRA, CARLA C.. Proteomic Analysis of Yeast Mutant RNA Exosome Complexes. JOURNAL OF PROTEOME RESEARCH, v. 12, n. 12, p. 5912-5922, DEC 2013. (10/51842-3)

MIGUEL, RODRIGO BERNARDI; DIVINA PETERSEN, PHILIPPE ALEXANDRE; GONZALES-ZUBIATE, FERNANDO A.; OLIVEIRA, CARLA COLUMBANO; KUMAR, NARESH; DO NASCIMENTO, RAFAEL RODRIGUES; PETRILLI, HELENA MARIA; DA COSTA FERREIRA, ANA MARIA. Inhibition of cyclin-dependent kinase CDK1 by oxindolimine ligands and corresponding copper and zinc complexes. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, v. 20, n. 7, p. 1205-1217, OCT 2015. (11/50318-1, 10/51842-3, 13/07937-8)

GATTI DA SILVA, GUILHERME H.; JURICA, MELISSA S.; CHAGAS DA CUNHA, JULIA P.; OLIVEIRA, CARLA C.; COLTRI, PATRICIA P.. Human RNF113A participates of pre-mRNA splicing in vitro. Journal of Cellular Biochemistry, v. 120, n. 5, p. 8764-8774, MAY 2019. (10/51842-3, 13/02738-7, 11/20664-5, 17/06994-9)